Murine Embryonic Stem Cell (mESC) Culture and Passaging
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Embryoid Body (EB) Preparation and Grem2 Treatment
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Results: Grem2-induction of Cardiomyocyte Differentiation
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Conclusion
副本
The overall goal of this differentiation protocol is to generate homogeneous populations of atrial cardiomyocytes in culture. So this method can help answer key questions in the study of cardiac development and cardiac diseases such as atrial arrh
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Generating cardiomyocytes from pluripotent stem cells in vitro allows access to large amounts of cardiac tissue in vitro for basic science and clinical applications. This protocol uses the atrializing factor Grem2 to both increase the numbers of cardiomyocytes obtained and to generate cardiomyocytes with an atrial phenotype.