August 21st, 2016
•We present a strategic plan and protocol for identifying non-coding genetic variants affecting transcription factor (TF) DNA binding. A detailed experimental protocol is provided for electrophoretic mobility shift assay (EMSA) and DNA affinity precipitation assay (DAPA) analysis of genotype-dependent TF DNA binding.
Related Videos
Light/dark Transition Test for Mice
Assay for Neural Induction in the Chick Embryo
Elevated Plus Maze for Mice
Dissection of Hippocampal Dentate Gyrus from Adult Mouse
An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example