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Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

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11:35 min

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August 21st, 2016

DOI :

10.3791/54093-v

August 21st, 2016

•

12,823 Views

Daniel E. Miller¹, Zubin H. Patel¹^,²^,³, Xiaoming Lu¹^,³, Arthur T. Lynch¹, Matthew T. Weirauch*¹^,⁴, Leah C. Kottyan*¹

¹Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital, ²Medical Scientist Training Program, University of Cincinnati, ³Immunology Graduate Program, University of Cincinnati, ⁴Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children's Hospital

章

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要約

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Functional Non coding Genetic Variants

Electrophoretic Mobility Shift Assay EMSA

DNA affinity Precipitation Assay DAPA

Regulatory Proteins

Pathways

B lymphoblastoid Cells

Phosphatase Inhibitor

Phenylmethanesulfonyl Fluoride PMSF

Nonidet P 40

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