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Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

12.9K Views

11:35 min

August 21st, 2016

DOI :

10.3791/54093-v

August 21st, 2016


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Keywords Functional Non coding Genetic Variants

Chapters in this video

0:05

Title

0:56

Preparation of Nuclear Lysate from Cultured Cells

3:19

Electrophoretic Mobility Shift Assay (EMSA)

6:56

DNA Affinity Purification Assay (DAPA)

9:37

Results: Non-coding Genetic Variants Affect Transcription Factor DNA Binding

11:04

Conclusion

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