Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 1
3:07
Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 2
4:31
Density-gradient Ultracentrifugation (DGUC) for HDL Purification: Part 3
6:05
Fast-protein Liquid Chromatography (FPLC) for HDL Purification
8:05
Results: Importance of DGUC to Procedure and Small RNA Library Analysis
9:24
Conclusion
Transcript
The overall goal of this protocol is to quantify small non-coding RNAs on purified lipoproteins. This protocol can help answer key questions in the lipoprotein field, such as defining lipoprotein small RNA signatures in health and disease, as well
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This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.
