Preparation of Media for Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)
1:34
SILAC-labeling and Amino Acid Incorporation Control
3:15
Lipid Droplet Isolation of SILAC-labeled Huh7.5 Cells
7:59
Analysis of Lipid Droplets Purity
8:53
Results: Qualitative and Quantitative Analysis of the Lipid Droplets
9:57
Conclusion
副本
The overall goal of isolating lipid droplets for quantitative mass spectrometry is to understand the function of these organelles, for example, in the replication of pathogens such as the hepatitis C virus. This method can answer key questions in
登录或开始您的免费试用以访问此内容
Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.
