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12:21 min
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November 27th, 2016
DOI :
November 27th, 2016
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此视频中的章节
0:05
Title
0:47
Transfection of Adherent HEK293S GnTI-cells for Thermostability Screen
2:24
Scintillation Proximity-based Thermostability Screen with Citalopram
4:18
Expression of the Human Serotonin Transporter in HEK293S GnTI-cells
5:24
Affinity-purification of the Serotonin Transporter for Immunization and Crystallization
7:12
Formation of Transporter-antibody Complexes and Separation by Size-exclusion Chromatography
8:21
Crystallization of Transporter-antibody Complexes by Hanging Drop
10:01
Results: Thermostabilization and Crystallization of the Serotonin Transporter in an Antidepressant-bound State
11:25
Conclusion
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