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12:21 min
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November 27th, 2016
DOI :
November 27th, 2016
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Title
0:47
Transfection of Adherent HEK293S GnTI-cells for Thermostability Screen
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Scintillation Proximity-based Thermostability Screen with Citalopram
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Expression of the Human Serotonin Transporter in HEK293S GnTI-cells
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Affinity-purification of the Serotonin Transporter for Immunization and Crystallization
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Formation of Transporter-antibody Complexes and Separation by Size-exclusion Chromatography
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Crystallization of Transporter-antibody Complexes by Hanging Drop
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Results: Thermostabilization and Crystallization of the Serotonin Transporter in an Antidepressant-bound State
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Conclusion
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