Construction of the HaDV2-free Cotton Bollworm Colony
2:02
Preparation of HaDV2-containing Liquid Fluids
3:28
Construction of the HaDV2-infected Cotton Bollworm Colony
4:57
Tissue Distribution of HaDV2
7:01
Bioassays Testing the Effect of HaDV2 on Host Development
9:36
Results: Characterization of Effects of Densovirus on Bollworms
10:44
Conclusion
副本
The overall goal of this protocol is to study the effect of a Densovirus, HaDV2, on its host, Cotton Bollworm. This method can help answer key questions in the virus host interaction field such as the effect a now path-standing viruses on their ho
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Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.