Preparation of Detergent-soluble and High-salt Extracts of Tissue-specific IFs
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Automated Tissue Lysis for RNA and IF Protein Extraction in High-volume Experiments
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Results: High-salt Extraction of Intermediate Filament Proteins Using Lysing Matrix
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Conclusion
副本
The overall goal of this biochemical procedure is to isolate intermediate filament enriched fractions from multiple mouse tissues in order to study post translational modifications of the different tissue specific intermediate filament proteins. T
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In this method, we present biochemical procedures for rapid and efficient isolation of intermediate filament (IF) proteins from multiple mouse tissues. Isolated IFs can be used to study changes in post-translational modifications by mass spectrometry and other biochemical assays.