Standardization of Neuronal Cultures Using Microfluidic Devices: Device Assambly
2:54
Plating Neurons in Microfluidic Systems
4:47
PDL-bead Adhesion and Micromanipulation
6:13
Pulling Neurites and Connecting Neurons
8:45
Results: The Newly Induced, Elongated, and Connected Neurite Can Transfer Information Between Two Isolated Neuronal Populations
9:39
Conclusion
副本
The overall goal of this procedure is to initiate new neurites, rapidly extend them, and precisely connect them to target cells. This procedure is only possible by using cells grown in a controlled and reproducible environment. This method allows
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This procedure describes how to rapidly initiate, extend and connect neurites organized in microfluidic chambers using poly-D-lysine-coated beads fixed to micropipettes that guide neurite elongation.