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Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

DOI :

10.3791/55836-v

June 3rd, 2017

June 3rd, 2017

10,800 Views

1Basic Sciences Division, Fred Hutchinson Cancer Research Center, 2Department of Biology, Indiana University

We describe chromatin endogenous cleavage coupled with high-throughput sequencing (ChEC-seq), a chromatin immunoprecipitation (ChIP)-orthogonal method for mapping protein binding sites genome-wide with micrococcal nuclease (MNase) fusion proteins.

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Keywords ChEC seq

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