Preparation of Primary Peripheral Blood Mononuclear Cells by Density Gradient Separation
3:15
Enrichment of B Cells Negative Selection
5:34
Development of the Xenograft Mouse Model
6:07
Chimerism Analysis of Tumor Cell Engrafted in Different Organs
7:43
Results: Use of Flow Cytometry to Analyze the B Cell Enrichment in Mice Xenografts Obtained from Mantle Cell Lymphoma Patients
9:08
Conclusion
Transcript
The overall goal of this method is to establish a xenograft mouse model for mantle cell lymphoma, to develop treatment strategies for this disease. This method can help answer key questions in the field of hematooncology such as therapeutic outcom
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Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
