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血液循环中稀有抗原特异 B 细胞的鉴别人单克隆抗体的生成

10.3K Views

13:14 min

February 6th, 2018

DOI :

10.3791/56508-v

February 6th, 2018


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132

此视频中的章节

0:04

Title

1:17

Isolation of Human Peripheral Blood Mononuclear Cells

2:00

Tetramer-Associated Magnetic Enrichment of Antigen Specific B-Cells

3:58

Staining of Antigen Specific Human B-Cells and Cell Sorting

4:56

Single Cell RT-PCR

6:00

Screening and Verification of Heavy and Light Chain Expressing Vectors

7:45

Production of Monoclonal Antibodies

10:33

Results: Detection of Relevant Antigen Specific B-Cells and Generation of Highly Discriminative Monoclonal Antibody from Donor Peripheral Blood Mononuclear Cells

12:41

Conclusion

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