Preparation of Barley Grains and Plasmid DNA for Bombardment
2:42
Particle Bombardment of Embryoless Barley Grains
4:42
Extraction of Soluble Protein from the Bombarded Grains
6:24
Measurement of GUS Activity from Grain Extracts
8:15
Results: Measurement of Gene Expression in Barley Aleurone Cells after Particle Bombardment
9:50
Conclusion
Transcript
The overall goal of this procedure is to measure gene expression from reporter constructs after introducing them into barley aleurone cells by particle bombardment. This method can help answer key questions in plant hormone regulated gene expressi
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An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure.
