Khan 博士承认来自 NIH/发育研究院 K12HD052892、Alpha-1 基金会、基金会和匹兹堡肝脏研究中心的赠款支持。Michalopoulos 博士承认来自 NIH/NIDDK P01DK096990 的赠款支持。我们感谢安妮的出色和慷慨的技术援助。我们还感谢大卫. 盖勒博士提供了进入移植/啮齿动物显微外科实验室的机会。

所有程序都是按照匹兹堡大学机构动物保育和使用委员会的道德准则进行的。
1. 基本技术和一般程序
<ol><li>所有的显微外科设备使用前。</li>
	<li>为了避免麻醉杀死老鼠, 仔细检查它的呼吸模式。</li>
	<li>尽可能少接触器官和肠道, 以避免损害器官和造成出血。用湿棉签或纱布海绵按压出血点。</li>
	<li>用湿棉签对器官进行温和的操作。湿的, 无纺布纱布海绵用于两个大小, 3.5 x 3.5 厘米和 6.0 x 6.0 厘米. 用小的来收回整个肝脏。用大一点的包包裹小肠和大肠。</li>
</ol>2. 术前准备
<ol><li>使用雄性 c57/b6 小鼠, 8-12 周的年龄 (当体重约20-35 克) 的部分胆管结扎。</li>
	<li>允许老鼠免费进入水和食物, 直到麻醉的诱导。</li>
</ol>3. 部分胆总管结扎术
<ol><li>麻醉把动物放在一个感应腔内, 混合 1.5-2.0% 异氟醚和1升/分氧。观察呼吸速率, 仔细监测动物。当动物的呼吸速率为每秒一次呼吸时, 老鼠被麻醉的很好。因为前面肢体撤退反射更加迅速地消失, 捏后肢脚并且证实完全缺乏撤退反射。眼睛应与眼科润滑剂润滑, 以防止角膜溃疡。注意, 第一剂量的镇痛可以预先手术, 以防止刺激疼痛受体。</li>
	<li>用发剪刀刮腹壁, 将鼠标放在手术显微镜下的手术台上。用胶带把四肢固定在桌子上。使用异氟醚吸入在全麻下维持小鼠。<ol>
<li>调整异氟醚蒸发器, 并使用 2.0-3.0% 异氟醚诱导。</li>
	</ol>
</li>
	<li>使用无菌拭子, 先用 0.3-0.5 毫升聚维酮碘对腹壁进行消毒, 其次采用70% 乙醇。动物应该用不育的褶皱覆盖。</li>
	<li>用剪刀和 Adson 钳从剑突到耻骨进行长中线腹部切口。做腹部切口后, 将异氟醚减少到 0.8-1.0% 进行维护。</li>
	<li>使用微型牵引器暴露腹腔。用蚊子钳握住剑突, 然后向鼠标头缩回。用软粘土固定好蚊虫钳。</li>
	<li>将1-2 毫升温热 (37 °c) 消毒的磷酸盐缓冲盐水 (PBS) 注入腹腔, 以避免损伤腹部器官。用湿棉签握住小肠, 轻轻地将它们缩回鼠标的头部。减少小肠和腹腔之间的韧带, 以避免伤害小肠 (图 1A)。</li>
	<li>用湿的无纺布海绵包裹小肠和大肠。把它们放在腹腔的尾侧。肠道包裹在无菌纱布可以放置在无菌覆盖的领域, 以避免污染。</li>
	<li>用一个小的湿无纺布海绵将肝脐和肝-十二指肠韧带 (图 1B) 轻轻地收回整个肝脏, 剑突。</li>
	<li>检查以确认肝门的解剖。胆总管位于门静脉的腹侧。确认右侧和左肝胆管的位置, 将其排入肝门的主胆汁汇合处。</li>
	<li>用缝合针的钝面 (图 2A) 确定左侧肝胆管引流左叶, 并用10-0 尼龙缝线将其包围。避免损伤门静脉、肝动脉和肝表面。</li>
	<li>用10-0 尼龙结扎左胆管。(图 2B)。</li>
	<li>用湿棉拭子将小肠和大肠放在手术前的相同位置。</li>
	<li>确认没有肠道扭转和腹腔内出血, 然后关闭肌肉和皮肤两层 4-0 vicryl。编织缝合 (Vicryl) 用于皮肤闭合, 但单丝更倾向于减少感染的可能性。 注: 关闭筋膜由剑突到耻骨的持续缝合, 并通过从耻骨到剑突的持续缝合来关闭皮肤。</li>
	<li>关闭切口后停止麻醉。</li>
</ol>4. 术后治疗及随访
<ol><li>手术后, 捏住老鼠的背皮, 注射 Buprenex (0.1 毫克/千克) 和头孢唑啉 (100 毫克/千克), 其次是 Buprenex 注射液每12小时3天, 每24小时头孢唑啉分别为3天11,12 ,13。注意, 第一剂量的镇痛可以预先手术, 以防止刺激疼痛受体。头孢唑啉和其他围手术期抗生素只需要免疫抑制动物, 如用于移植研究。 注: 如果手术成功, 老鼠通常会在30分钟内痊愈, 而老鼠的活动与术前情况差不多。</li>
	<li>每天检查老鼠是否有任何遇险迹象。<ol>
<li>在常规麻醉下, 通过颈椎脱位牺牲小鼠, 在 pBDL 后, 在规定的时间点 (即, 7, 14, 21 天) 获得肝脏和其他组织样本。</li>
	</ol>
</li>
</ol>

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作者没有披露。

虽然 cBDL 是阻塞性胆汁淤积症最常见的实验技术, 但它可以在啮齿动物中提出多种挑战。根据完成终点所需的术后天数, 随着时间的推移, 动物的发病率和死亡率会显著下降。在一些转基因小鼠肝病模型的作用下, 组织损伤和胆道坏死的程度进一步恶化, 表现为肝实质异常和基线功能。与 cBDL 相比, pBDL 的报道是有限的, 但这种技术可以作为选择实验的替代品, 以减少负面结果和动物损失。
<p class="j...

外科模型的急性肝损伤和修复, 如部分肝切除或胆总管结扎 (cBDL), 已被用于研究肝再生和病理1,2。在 cBDL, 胆总管 (排泄所有肝脏产生的胆汁) 结扎, 导致梗阻性胆汁淤积, 炎症和纤维化在手术后的前2-3 周, 最终进展为肝硬化3。胆管增生, 转分化和诱导肝祖细胞也是 cBDL4,5的特征。虽然 cBDL 阐明了阻塞性 cholangiopathy 的具体机制, 其特点是在野生型小鼠中具有可重现的结果, 在这些菌株与正常肝脏6,7,8,在某些转基因小鼠肝脏疾病模型中, 由于胆道梗阻的发病率和死亡率随着时间的推移而增加, 需要的动物比预期的要多。这项技术也有利于研究具有潜在肝病的转基因小鼠, 否则可能无法容忍 cBDL 后长时间课程实验的压力。
pBDL 可以提供一个合理的替代方案来帮助克服其中的一些挑战。野生型小鼠的初步 pBDL 研究旨在区分炎症的局部作用和系统性介质9。在 pBDL 中, 胆总管上方右、左肝胆管的主胆汁汇合处仔细地可视于脐, 只结扎左肝胆管, 产生局部梗阻性胆汁淤积。pBDL 为 cBDL 提供了一些优势。在 pBDL 中, unligated 右叶作为结扎左叶的一个相同的内部控制, 受到相同的系统性影响, 如营养状况或循环因素。虽然全身性的炎症介质存在, 大泽et等) 观察到较少的纤维化和坏死的 unligated 裂片, 但增加炎症细胞和转化生长因子β表达在结扎叶 9.
最近, pBDL 被重新定义为研究保留胆汁的肝脏实质作用10。通过使用同样的动物进行内部控制 (unligated "假") 和实验组, pBDL 有效地将每项实验所需的动物数量减半, 这反过来又更具成本效益。小鼠对 pBDL 的耐受效果好得多, 在结扎叶中可实现理想的时间过程实验 (≥2周)。最重要的是, pBDL 可以区分梗阻性胆汁淤积症和保留胆汁成分的直接肝内效应, 与 unligated 控制叶相比。总的来说, pBDL 是研究肝局部胆汁淤积症叶特异效应的一种很有吸引力的方法。

<table><tbody><tr><td>Microscope-Leica Wild M650, 6&amp;ndash;40&amp;times; magnification</td><td>Leica Microsystems</td><td>n/a</td><td></td></tr><tr><td>Tec 3 isoflurane funnel fill vaporizer</td><td>General Anesthetic Services</td><td>n/a</td><td></td></tr><tr><td>Stevens Tenotomy Scissors</td><td>Accurate Surgical &amp;amp; Scientific Instruments Corporation</td><td>ASSI.9193</td><td></td></tr><tr><td>Adson Forceps</td><td>Fine science tools</td><td>11006-12</td><td></td></tr><tr><td>Mosquito classic delicate hemostatic forceps</td><td>Codman</td><td>30-4472</td><td></td></tr><tr><td>Micro-retractor</td><td>Murdock; Roboz Surgical Instrument</td><td>RS-6550</td><td></td></tr><tr><td>Nonwoven gauze sponges</td><td>Fisherband</td><td>870-PC-DBL</td><td></td></tr><tr><td>Puritan 6&amp;rdquo; Small Cotton Swab w/Wooden Handle</td><td>Puritan Medical Products Company</td><td>868-WCS</td><td></td></tr><tr><td>Dumont SS Forceps - Standard Tips/Straight/Inox/13.5cm</td><td>Fine science tools</td><td>11203-23</td><td></td></tr><tr><td>Dumont SS Forceps - Standard Tips/Angled 45&amp;deg;/Inox/13.5cm</td><td>Fine science tools</td><td>11203-25</td><td></td></tr><tr><td>Vannas Spring Scissors - 3mm Cutting Edge</td><td>Fine science tools</td><td>15000-00</td><td></td></tr><tr><td>Microneedle holder</td><td>Aesculap Surgical Instruments</td><td>FD231R</td><td></td></tr><tr><td>Micro AROSuture, sterile 10-0 nylon suture, 70 &amp;micro;m, TAP points</td><td>AROSurgical Instruments Corporation</td><td>T4A10N07</td><td></td></tr><tr><td>4-0 Vicryl</td><td>Ethicon</td><td>J662H</td><td></td></tr><tr><td>5-ml Syringe</td><td>BD</td><td>309646</td><td></td></tr><tr><td>Falcon tissue culture dish, 60 &amp;times; 15 mm</td><td>Corning</td><td>353002</td><td></td></tr><tr><td>Isoflurane, United States Pharmacopeia (USP) liquid for inhalation, 250 ml</td><td>Piramal Healthcare</td><td>NDC 66794-017-25</td><td></td></tr><tr><td>Buprenex injectable (buprenorphine hydrochloride)</td><td>Reckitt Benckiser Pharmaceuticals</td><td>NDC 12496-0757-1</td><td></td></tr><tr><td>Cefazolin for injection , USP</td><td>APP Pharmaceuticals</td><td>NDC 63323-238-61</td><td></td></tr><tr><td>PVP scrub solution (povidone&amp;ndash;iodine 7.5%)</td><td>Medline</td><td>NDC 12496-0757-1</td><td></td></tr><tr><td>70% Ethanol</td><td>Decon Labs</td><td>2716</td><td></td></tr><tr><td>Phosphate Buffered Saline Without Calcium or Magnesium</td><td>LONZA</td><td>17-516F</td><td></td></tr><tr><td>Sirius Red</td><td>Sigma</td><td>365548</td><td></td></tr><tr><td>Hematolxylin</td><td>Fisher (Richard-Allan Scientific)</td><td>22-050-111 (7211)</td><td></td></tr><tr><td>Eosin</td><td>Anatech (Fisher)</td><td>832 (NC9686037)</td><td></td></tr><tr><td>Formalin</td><td>Fisher</td><td>SF100-20</td><td></td></tr></tbody></table>

partial bile duct ligation in the mouse: a controlled model of localized obstructive cholestasis

在啮齿动物中, 胆总管完全胆管结扎术 (cBDL) 是研究梗阻性胆汁淤积症和胆管增生的一种成熟的外科技术。然而, 长期实验可能导致发病率和死亡率的增加。在选择有潜在肝病的小鼠菌株中, 甚至可以通过结扎肝单叶来进行有意义的比较, 这样可以减少动物的损失和开支。在这里, 我们描述的部分胆管结扎 (pBDL) 在小鼠, 其中只有左肝胆管结扎, 导致胆道梗阻的左叶, 而不是其余的裂片。通过仔细的显微外科技术, pBDL 实验可以是成本效益, 因为 unligated 叶作为一个内部控制的结扎裂片, 当受到相同的条件在同一动物。不像 cBDL, 一个单独的假操作控制组是不必要的。pBDL 是非常有用的直接比较局部和系统性的影响胆汁淤积症和其他保留胆汁成分。pBDL 也可以作为一种新的方法来研究与药物和细胞迁移有关的机制。

对于这一典型的实验, pBDL 在三 PiZ 转基因小鼠中进行, 概括了 alpha-1 胰岛素 (A1AT) 缺乏中发现的人肝病。PiZ 小鼠 overexpresses 一种转基因, 由 DNA 的多个基因组片段组成, 其中包含突变体的人类 A1AT 基因和启动子的编码区域, 以及上游和下游侧翼区域的 2 kilobases14, 15。正常 A1AT 是一种分泌的血清蛋白在肝脏中产生。A1AT 的突变体产生?...

Watch this Scientific Journal Video about Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis at JoVE.com

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis

在这里, 我们提出了部分胆管结扎作为一个外科模型的肝损伤和再生的啮齿动物。

小鼠部分胆管结扎术: 局部梗阻性胆汁淤积症的对照模型

In rodents, complete bile duct ligation (cBDL) of the common bile duct is an established surgical technique for studying obstructive cholestasis and bile ...

partial-bile-duct-ligation-mouse-controlled-model-localized

Research

JoVE Journal

Biology

15.4K 次观看.  Jichi Medical University. 在这里, 我们提出了部分胆管结扎作为一个外科模型的肝损伤和再生的啮齿动物。

视频: Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis

Creation of Reversible Cholestatic Rat Model

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Cholestasis can cause various physiological changes and affect the nutritional status and surgical outcomes. Study of the pathophysiological changes occurring in the liver and other organs is of importance. Various studies have been done in cholestatic rat models.
We used a reversible cholestatic rat model in our recent study looking at the role of methylprednisolone in the ischemia reperfusion injury. Various techniques for creation of a reversible cholestatic model have been described. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model.
This model can be used in various studies, such as looking at the changes in nutritional, physiological, pathological, histological and immunological changes in the gastrointestinal tract. This model can also be used to see the effects of cholestasis and various therapeutic interventions on major hepatic surgeries.

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model.

可逆的胆汁淤积大鼠模型的建立

Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Cholestasis can cause various physiological changes and ...

科研

医学

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration

Morphological organ regeneration following acute tissue loss is common among lower vertebrates, but is rarely observed in mammalian postnatal life. Adult liver regeneration after 70% partial hepatectomy results in hepatocyte hypertrophy with some replication in remaining lobes with restoration of metabolic activity, but with permanent loss of the injured lobe&#39;s morphology and architecture. Here, we detail a new surgical method in the neonate that leaves a physiologic environment conducive to regeneration. This model involves amputation of the left lobe apex and a subsequent conservative management regimen, and lacks the necessity for ligation of major liver vessels or chemical injury, leaving a physiologic environment where regeneration may occur. We extend this protocol to amputations on juvenile (P7-14) mice, during which the injured liver transitions from organ regeneration to compensatory growth by hypertrophy. The presented, brief 30 min protocol provides a framework to study the mechanisms of regeneration, its age-associated decline in mammals, and the characterization of putative hepatic stem or progenitors.

Here, we present a new method for partial resection of the left hepatic lobe in neonatal (day 0) mice. This new protocol is suitable for studying acute liver injury and injury response in the neonatal setting.

部分小叶切除术: 形态学肝再生的外科模型

Morphological organ regeneration following acute tissue loss is common among lower vertebrates, but is rarely observed in mammalian postnatal life. Adult ...

发育生物学

Determining Bile Duct Density in the Mouse Liver

Mouse is broadly used as a model organism to study biliary diseases. To evaluate the development and function of the biliary system, various techniques are used, including serum chemistry, histological analysis, and immunostaining for specific markers. Although these techniques can provide important information about the biliary system, they often do not present a full picture of bile duct (BD) developmental defects across the whole liver. This is in part due to the robust ability of the mouse liver to drain the bile even in animals with significant impairment in biliary development. Here we present a simple method to calculate the average number of BDs associated with each portal vein (PV) in sections covering all lobes of mutant/transgenic mice. In this method, livers are mounted and sectioned in a stereotypic manner to facilitate comparison among various genotypes and experimental conditions. BDs are identified via light microscopy of cytokeratin-stained cholangiocytes, and then counted and divided by the total number of PVs present in liver section. As an example, we show how this method can clearly distinguish between wild-type mice and a mouse model of Alagille syndrome. The method presented here cannot substitute for techniques that visualize the three-dimensional structure of the biliary tree. However, it offers an easy and direct way to quantitatively assess BD development and the degree of ductular reaction formation in mice.

We present a rather simple and sensitive method for accurate quantification of bile duct density in the mouse liver. This method can aid in determining the effects of genetic and environmental modifiers and the effectiveness of potential therapies in mouse models of biliary diseases.

确定小鼠肝脏中的胆汁密度

Mouse is broadly used as a model organism to study biliary diseases. To evaluate the development and function of the biliary system, various techniques ...

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

In most vertebrates, the liver produces bile that is necessary to emulsify absorbed fats and enable the digestion of lipids in the small intestine as well as to excrete bilirubin and other metabolic products. In the liver, the experimental obstruction of the extrahepatic biliary system initiates a complex cascade of pathological events that leads to cholestasis and inflammation resulting in a strong fibrotic reaction originating from the periportal fields. Therefore, surgical ligation of the common bile duct has become the most commonly used model to induce obstructive cholestatic injury in rodents and to study the molecular and cellular events that underlie these pathophysiological mechanisms induced by inappropriate bile flow. In recent years, different surgical techniques have been described that either allow reconnection or reanastomosis after bile duct ligation (BDL), e.g., partial BDL, or other microsurgical methods for specific research questions. However, the most frequently used model is the complete obstruction of the common bile duct that induces a strong fibrotic response after 21 to 28 days. The mortality rate can be high due to infectious complications or technical inaccuracies. Here we provide a detailed surgical procedure for the BDL model in mice that induce a highly reproducible fibrotic response in accordance to the 3R rule for animal welfare postulated by Russel and Burch in 1959.

Disruption of bile flow results in severe inflammatory cholestatic liver injury with a characteristic time-dependent sequence of morphological alterations. Here we present a protocol for the surgical ligation of the common bile duct in mice that allows to induce a strong fibrotic response after 21 to 28 days.

胆管结扎小鼠:感应炎性肝损伤和纤维化的阻塞性胆汁淤积

In most vertebrates, the liver produces bile that is necessary to emulsify absorbed fats and enable the digestion of lipids in the small intestine as well ...

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

Biliary atresia (BA) is a fatal disease involving obstructive jaundice, and it is the most common indication for liver transplantation in children. Due to the complex etiology and unknown pathogenesis, there are still no effective drug treatments. At present, the classic BA mouse model induced by rhesus rotavirus (RRV) is the most commonly used model for studying the pathogenesis of BA. This model is characterized by growth retardation, jaundice of the skin and mucosa, clay stools, and dark yellow urine. The histopathology shows severe liver inflammation and obstruction of the intrahepatic and extrahepatic bile ducts, which are similar to the symptoms of human BA. However, the livers of end-stage mice in this model lack fibrosis and cannot fully simulate the characteristics of liver fibrosis in clinical BA. The presented study developed a novel BA mouse model of chronic liver fibrosis by injecting 5-10 &#181;g of anti-Ly6G antibody four times, with gaps of 2 days after each injection. The results showed that some of the mice successfully formed chronic BA with typical fibrosis after the time lapse, meaning these mice represent a suitable animal model for the virus-induced liver fibrosis mechanistic study of BA and a platform for developing future BA treatments.

We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments.

慢性肝纤维化小鼠模型用于研究胆道闭锁

Biliary atresia (BA) is a fatal disease involving obstructive jaundice, and it is the most common indication for liver transplantation in children. Due to ...

免疫与感染

A Mouse Model for Chronic Pancreatitis via Bile Duct TNBS Infusion

Chronic pancreatitis (CP) is a complex disease involving pancreatic inflammation and fibrosis, glandular atrophy, abdominal pain and other symptoms. Several rodent models have been developed to study CP, of which the bile duct 2,4,6 -trinitrobenzene sulfonic acid (TNBS) infusion model replicates the features of neuropathic pain seen in CP. However, bile duct drug infusion in mice is technically challenging. This protocol demonstrates the procedure of bile duct TNBS infusion for generation of a CP mouse model. TNBS was infused into the pancreas through the ampulla of Vater in the duodenum. This protocol optimized drug volume, surgical techniques, and drug handling during the procedure. TNBS-treated mice showed features of CP as reflected by bodyweight and pancreas weight reductions, changes in pain-associated behaviors, and abnormal pancreatic morphology. With these improvements, mortality associated with TNBS injection was minimal. This procedure is not only critical in generating pancreatic disease models but is also useful in local pancreatic drug delivery.

Chronic pancreatitis (CP) is a disease characterized by inflammation and fibrosis of the pancreas, often associated with intractable abdominal pain. This article focuses on refining the technique to generate a mouse model of CP via bile duct infusion with 2,4,6 -trinitrobenzene sulfonic acid (TNBS).

通过胆管 TNBS 输液治疗慢性胰腺炎的鼠标模型

Chronic pancreatitis (CP) is a complex disease involving pancreatic inflammation and fibrosis, glandular atrophy, abdominal pain and other symptoms. ...

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

Obesity is a major health issue worldwide. As a response, bariatric surgeries have emerged to treat obesity and its related comorbidities (e.g., diabetes mellitus, dyslipidemia, non-alcoholic steatohepatitis, cardiovascular events, and cancers) through restrictive and malabsorptive mechanisms. Understanding the mechanisms by which these procedures allow such improvements often require their transposition into animals, especially in mice, because of the ease of generating genetically modified animals. Recently, the single-anastomosis duodeno-ileal bypass with sleeve gastrectomy (SADI-S) has emerged as a procedure that uses both restrictive and malabsorptive effects, which is being used as an alternative to gastric bypass in case of major obesity. Thus far, this procedure has been associated with strong metabolic improvements, which has led to a marked increase in its use in daily clinical practice. However, the mechanisms underlying these metabolic effects have been poorly studied as a result of a lack of animal models. In this article, we present a reliable and reproducible model of SADI-S in mice, with a special focus on perioperative management. The description and use of this new rodent model will be helpful for the scientific community to better understand the molecular, metabolic, and structural changes induced by the SADI-S and to better define the surgical indications for clinical practice.

Single-anastomosis duodeno-ileal bypass (SADI-S) is an emerging bariatric procedure with important metabolic effects. In this article, we present a reliable and reproducible model of SADI-S in mice.

小鼠单吻合十二指肠回肠旁路与袖状胃切除术模型

Obesity is a major health issue worldwide. As a response, bariatric surgeries have emerged to treat obesity and its related comorbidities (e.g., diabetes ...

<meta charset="utf8"></head><body>在分期肝切除术动物模型中研究 ALLPS 的肝脏再生

A Mouse Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Procedure Aided by Microscopy

Hepatectomy is widely regarded as the primary treatment for hepatic malignancies; yet, postoperative liver failure remains a major cause of perioperative mortality, severely impacting patient outcomes. In a robust hepatic environment, the future liver remnant (FLR) must exceed 25%, and in cases of cirrhosis, this requirement increases to over 40%. The inadequacy of FLR is currently a major obstacle in the progression of hepatic surgery.
Traditional methods to enhance FLR hypertrophy mainly focus on portal vein embolization (PVE), but its effectiveness is considerably limited. In recent years, there have been numerous reports on a novel biphasic hepatectomy method involving hepatic partitioning and portal vein ligation, known as associating liver partition and portal vein ligation for staged hepatectomy (ALPPS). ALPPS surpasses PVE in efficiently and considerably inducing FLR hypertrophy. However, the detailed mechanisms driving ALPPS-facilitated hepatic regeneration are not fully understood. Thus, replicating ALPPS in animal models is crucial to thoroughly investigate the molecular mechanisms of hepatic regeneration, offering valuable theoretical and practical insights.

<meta charset="utf8"></head><body>作者聚焦：通过 ALPPS 小鼠模型推进肝脏再生研究

Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) has been shown to result in remarkable liver regeneration after several weeks post first-stage surgery. Animal models that have undergone ALPPS have been developed to explore potential liver regeneration ability and achieve favorable clinical outcomes.

显微镜辅助分期肝切除术的关联肝分区和门静脉结扎的小鼠模型

Hepatectomy is widely regarded as the primary treatment for hepatic malignancies; yet, postoperative liver failure remains a major cause of perioperative ...

小鼠部分胆管结扎术: 局部梗阻性胆汁淤积症的对照模型

摘要

探索更多视频

此视频中的章节

可逆的胆汁淤积大鼠模型的建立

部分小叶切除术: 形态学肝再生的外科模型

确定小鼠肝脏中的胆汁密度

胆管结扎小鼠:感应炎性肝损伤和纤维化的阻塞性胆汁淤积

慢性肝纤维化小鼠模型用于研究胆道闭锁

通过胆管 TNBS 输液治疗慢性胰腺炎的鼠标模型

小鼠单吻合十二指肠回肠旁路与袖状胃切除术模型

显微镜辅助分期肝切除术的关联肝分区和门静脉结扎的小鼠模型

显微镜辅助分期肝切除术的关联肝分区和门静脉结扎的小鼠模型

显微镜辅助分期肝切除术的关联肝分区和门静脉结扎的小鼠模型