这项工作得到了 Kaken (17H05939; 17H05551)、臣基金会和 Suzuken 纪念基金会的支持。

所有涉及动物的程序都是根据 NIH 的动物保护和使用指南进行的。
1. 集成微驱动器阵列的研制
<ol><li>为皮质 LFP 录制创建微驱动器阵列, 如其他地方所述1,2,3。在电极接口板 (EIB) 上留出至少6个金属孔, 用于心电图/肌电图/BR 通道, 如1.2 所述, 连接到 bioflex 线。</li>
	<li>将 bioflex 线切成6片, 长度为5.0 厘米. 剥去聚四氟乙烯 (PTFE) 涂布两端的所有金属丝件, 长度为5.0 毫米. 将每个金属丝件的一端连接到一个打开的金属孔 (ECG/肌电信号/BR 通道) 上。 有金别针的欧洲投资银行。</li>
	<li>将搪瓷线切成两5.0 厘米的碎片。焊锡每根导线的一端到欧洲投资银行的地面/参考 (g/r) 通道上 (图 3, 另请参阅以前的论文12,13)。</li>
	<li>为制备心电图电极, 将 bioflex 线切成两个16厘米的片。将这些金属丝片两端的聚四氟乙烯涂层剥离在一端 (短端) 的长度为5.0 毫米, 另一端为15毫米 (长尾)。</li>
	<li>形成一个直径为2.0 毫米的钢丝圈, 弯曲长尾线, 并通过焊接固定环的形状。</li>
	<li>为制备肌电图电极, 将 bioflex 线切成2片, 长度为8厘米. 从这些金属丝件两端剥离聚四氟乙烯涂层, 长度为5.0 毫米。</li>
	<li>为制备 BR 电极, 将 bioflex 线切割成2件, 长度为6.0 厘米. 剥离这些金属丝件两端的搪瓷涂层, 长度为5.0 毫米. 每根焊丝的一端焊在不锈钢螺钉的头上 (阀杆直径米: 1.0 毫米, 茎长: 4.0 毫米)。</li>
	<li>为制备接地/参考 (gr) 电极, 将搪瓷丝切成2片, 长度为6.0 厘米. 剥去这些金属丝件两端的搪瓷涂层, 长度为5.0 毫米. 焊料的每一个一端的这些电线件的头一个不锈钢 st鳗鱼螺杆 (茎直径: 1.4 毫米, 茎长: 3.0 毫米)。</li>
	<li>气体杀菌所有电极和不锈钢螺丝, 并保持在一个干净的空间。</li>
</ol>2. 心电图/肌电图电极植入术
注: 使用灭菌手套和蒸压器具进行无菌技术的所有手术步骤。对于所有的步骤涉及创建切口, 消毒皮肤与70% 乙醇之前, 并覆盖切口与手术窗帘。
<ol><li>固定在一个扁平的热垫上的麻醉 (1.0-3.0% 异氟烷气体) 鼠背部。给丁丙诺啡止痛。将兽医软膏放在老鼠的眼睛上, 防止干燥。使用 betadine 清洁皮肤表面。</li>
	<li>在内侧胸区切开2.0 厘米。通过分离胸肌来暴露肋间肌肉。将心电图电极的环缝合至肋间肌。</li>
	<li>把动物的胃固定在热垫上。在背颈区域做一个1.0 厘米的切口。</li>
	<li>插入心电图电极皮下通过胸部切口。将两端滑动到背颈区域, 从颈部切口拉出。缝合胸部切口。</li>
	<li>将每一个肌电图电极的一端插入到长度为2.0 厘米的颈部切口。通过缝合将肌电信号电极固定在颈部肌肉上。</li>
</ol>3. 集成微驱动器阵列和 BR 电极的植入
<ol><li>把老鼠固定在一个立体定位装置上。沿着中线从眼睛到颈部区域, 在头上做一个3.0 厘米的切口。揭露头骨</li>
	<li>使两个圆形开颅直径 0.7-1.0 毫米以上的嗅球11.0 毫米前和1毫米双边 bregma 与高速演习。在头骨中植入两个 BR 电极, 直到螺钉茎的尖端附着在脑表面。</li>
	<li>使两个圆形开颅直径 0.7-1.0 毫米以上的额叶2.7 毫米前和2.7 毫米双边到 bregma。将两个克/r 电极植入头骨, 直到螺钉茎尖端附着在脑表面。</li>
	<li>使六到八孔直径1.0 毫米的区域周围的大开颅手术。植入锚螺钉 (茎直径: 1.4 毫米, 茎长: 3.0 毫米) 在头骨。</li>
	<li>做一个大圆形开颅手术, 直径为2.0 毫米以上的海马3.8 毫米后, 2.5 毫米双边到 bregma。放置集成的微驱动器阵列, 使驱动器阵列的套管尖端位于大型开颅手术上方。</li>
	<li>用100µL 两种溶液, 即 0.5% (按质量) 海藻酸钠和 10% (按质量) 氯化钙, 填补套管尖端与脑表面之间的间隙空间. 注: 这一过程形成一个透明凝胶在〜5分钟后, 两个解决方案是混合在头骨上。</li>
	<li>覆盖套管, BR 电极, g-/r 电极, 和锚螺钉与牙科水泥的厚度为0.5 厘米. 小心不要在这个步骤中用水泥覆盖 BR 和 g-/r 电极的开端。</li>
	<li>将心电图、肌电图、BR 和 g-/r 电极的开端焊接到以前连接到欧洲投资银行的个别导线提示上 (见步骤1.2 和 1.3)。</li>
	<li>覆盖底部的集成微驱动器阵列, 和所有电极线, 与牙科水泥。确保所有电极线均为完全覆盖, 以便大鼠在植入后无法将其刮出。</li>
	<li>在恢复足够的意识, 以保持胸骨卧床, 返回的动物到其透明的有机玻璃家庭 cag, 并保持它自己的自由获得水和食物。手术后, 用抗生素 (庆大霉素) 治疗动物。</li>
	<li>手术后, 每天观察动物。检查它们是否正常行走, 当实验者接触到微驱动器阵列时, 它们不会吱吱响。</li>
</ol>4.体内记录
注: 所有信号都放大, 采样在2赫, 带通滤波 (0.1-500 Hz), 除了单位活动 (取样在30赫和带通过滤 (500-6 赫))。
<ol><li>将集成的微驱动器阵列的 EIB 连接到记录设备的 headstage。</li>
	<li>在手术后的几周内拧紧螺钉, 以提高 tetrodes。一旦 tetrodes 与目标大脑区域相邻, 在数天的时间内将 tetrodes 固定在区域内, 以便稳定记录。</li>
	<li>当动物在录音室中自由移动时, 监控电信号。</li>
</ol>

<ol><li>Kloosterman, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Micro-drive+Array+for+Chronic+in+vivo+Recording%3A+Drive+Fabrication%5BTitle%5D)+AND+%22JoVE%22%5BJournal%5D)">Micro-drive Array for Chronic in vivo Recording: Drive Fabrication.</a> JoVE. (26), e1094(2009).</li>
<li>Nguyen, D. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Micro-drive+Array+for+Chronic+in+vivo+Recording%3A+Tetrode+Assembly%5BTitle%5D)+AND+%22Journal+of+Visualized+Experiments+%3A+JoVE%22%5BJournal%5D)">Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly.</a> Journal of Visualized Experiments : JoVE. (26), e1098(2009).</li>
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作者没有什么可透露的。

为了了解大脑如何调节外围活动水平, 反之亦然, 大规模的记录方法同时捕获多体区域的电 biosignals 是必要的。这项研究描述了一个手术程序, 一个记录系统, 监测大脑局部磁场电位, 心率, 肌肉结构的大小和呼吸率, 已经改善了记录系统, 用于脑组织中的细胞外记录。该系统在集成的微驱动器阵列上收集来自大脑和外围器官的电信号到单一的 EIB 上。综合微驱动器阵列的制备应在手术前至少几个小时?...

中枢神经系统控制身体状态以应对各种环境变化, 这种控制通常表现为心率、呼吸率和肌肉收缩的变化。然而, 很少有研究证实了这种外围生理因素与皮质活动有什么关联。为解决这一问题, 有必要从中央和外围组织监测电 biosignals 的大规模记录方法。在大脑皮层 , 局部场电位 ( LFP ) 信号入到皮质组织中的电极记录 extracellularly1,2,3。为了同时记录来自小哺乳动物 (如大鼠和小鼠) 皮质区域的多个 LFP 信号, 许多研究开发出了各种类型的定制电极组件, 称为微驱动器。传统的微驱动器由连接到电极中间部分的金属螺钉 (通常是 tetrodes), 一个容纳螺钉和电极的核心机构, 以及一个可容纳金属孔的电子接口板 (EIB) 来连接电极的打开端 (图 1、图 2和图 3)。这种电极组件使操作者能够控制在数天到数周内插入大脑的许多电极的深度, 并允许进行长期的神经元活动记录, 因为动物受到各种行为任务。在外围器官中, 心跳信号被植入在心脏区域或周围的一对电极上 (心电图), 在4,5,6, 和骨骼肌肉信号被记录作为 electromyograms (EMGs) 与插入到肌肉组织的电极7,8,9。用单单位录制10、11, 研究了嗅球电信号与呼吸 (BR) 节律之间的关系。在传统的记录系统中, 来自不同组织的这些信号被独立的记录装置捕获, 这意味着需要额外的实验系统来精确同步这些多台设备, 同时脑体信号的录音。这一制度是为了克服这一问题而制定的。在该系统中, 所有从外围器官记录的电信号, 包括心电图、EMGs 和反映呼吸节律的嗅球发出的电信号, 都集成到单个微驱动器阵列1,2 ,3, 这里称为一个集成的微驱动器阵列。该系统只需要一个多通道记录设备, 适用于任何常规的微驱动器阵列。这种技术的优点是它不需要任何特殊的设备或触发信号来匹配多个设备的记录时间, 而且它允许更方便的数据处理, 因为所有的信号都被记录为类似的数据类型。这项技术将有助于了解中央和外围器官之间的神经关联。本文介绍了与该技术相关的程序, 并给出了从大鼠获得的代表性数据集。

<table><tbody><tr><td>FEP Hookup Wire Stranded Stainless Steel&amp;nbsp;</td><td>Cooner Wire Company, Chatsworth, CA</td><td>AS 633</td><td>Bioflex wire</td></tr><tr><td>EIB-36-PTB</td><td>Neuralynx, Inc., Bozeman, MT</td><td>EIB-36-PTB</td><td>EIB</td></tr><tr><td>Cereplex&amp;nbsp; M</td><td>Blackrock&amp;nbsp; Microsystems, Salt Lake City, UT</td><td></td><td>Digital headstage</td></tr><tr><td>Cereplex Direct&amp;nbsp;</td><td>Blackrock&amp;nbsp; Microsystems, Salt Lake City, UT</td><td></td><td>Data acquisition system</td></tr><tr><td>UEW polyurethane magnet wire</td><td>Oyaide.com, Tokyo, Japan</td><td>UEW 0.14mm 20m&amp;nbsp;</td><td>Enamel wire</td></tr><tr><td>SD-102</td><td>Narishige, Tokyo, Japan</td><td>SD-102</td><td>High-speed drill</td></tr><tr><td>Minimo ONE SERIES ver.2</td><td>Minitor Co.,Ltd, Tokyo, Japan</td><td>C2012</td><td>High-peed drill Power Supply&amp;nbsp;</td></tr><tr><td>Provinice 250 mL</td><td>Shofu Inc., Kyoto, Japan</td><td>213620136</td><td>Dental cement</td></tr><tr><td>Small Animal Anesthetizer&amp;nbsp;</td><td>Biomachinery, Chiba, Japan</td><td>TK-7</td><td>Anesthetizer&amp;nbsp;</td></tr><tr><td>Buprenorphine hydrochloride</td><td>Sigma-Aldrich, St. Louis, MO</td><td>B7536-1ML</td><td>Analgesic</td></tr><tr><td>Isoflurane</td><td>DS Pharma Animal Health, Osaka, Japan&amp;nbsp;</td><td>Isoflu 250mL</td><td></td></tr><tr><td>Vaseline, White&amp;nbsp;</td><td>Wako Pure Chemical Industries, Ltd., Osaka, Japan</td><td>224-00165&amp;nbsp;</td><td>Vet ointment&amp;nbsp;</td></tr><tr><td>&amp;nbsp;Sodium alginate</td><td>Nacalai tesque, Kyoto, Japan</td><td>31131-85</td><td></td></tr><tr><td>Calcium Chloride Dihydrate</td><td>Wako Pure Chemical Industries, Ltd., Osaka, Japan</td><td>031-00435&amp;nbsp;</td><td></td></tr><tr><td>Stainless steel screw M1.0&amp;times;4.0&amp;nbsp;</td><td>MonotaRO, Hyogo, Japan</td><td>42617504</td><td>Stainless steel screw for BR electrodes</td></tr><tr><td>Stainless steel screw M1.4&amp;times;3.0</td><td>MonotaRO, Hyogo, Japan</td><td>42617687</td><td>Stainless steel screw for g/r electrodes and anchors</td></tr></tbody></table>

simultaneous recordings of cortical local field potentials, electrocardiogram, electromyogram, and breathing rhythm from a freely moving rat

监测大脑和周围组织的生理动态是解决一些问题, 关于大脑如何控制身体功能和内部器官节律当动物暴露在情绪挑战和变化的生活环境。在一般实验中, 来自不同器官的信号, 如大脑和心脏, 是由独立的记录系统记录的, 需要多个记录设备和处理数据文件的不同程序。本研究描述了一种新的方法, 可以同时监测电 biosignals, 包括在多个脑区的局部场电位, 代表心脏节律的心电图, electromyograms 代表清醒/睡眠相关的肌肉收缩, 呼吸信号, 在一个自由移动的老鼠。这种方法的记录配置是基于一个传统的微驱动器阵列, 用于皮层局部场电位记录, 其中有数以万计的电极, 并且从这些电极获得的信号被集成到单个电板安装在动物的头上。在这里, 这个录音系统得到了改进, 使外围器官的信号也被转移到一个电子接口板上。在单个手术中, 电极首先分别植入适当的身体部位和靶脑区。所有这些电极的开端, 然后焊接到在动物的头部以上的电气板的个别通道, 以便所有的信号可以集成到单一的电路板。将此主板连接到记录设备, 可以将所有信号收集到单个设备中, 从而降低了实验成本, 简化了数据处理, 因为所有数据都可以在同一数据文件中处理。这项技术将有助于了解中央和外围器官之间的神经关联。

这种方法可以同时捕获来自多个器官的生物电信号, 这些信号代表大脑的神经元活动, 心率, 呼吸节律和骨骼肌收缩(图 1)。图 4提供了可自由移动的大鼠在矩形框 (25 x 40 厘米2) 中自由觅食的具有代表性的记录数据。示例数据集包括移动和静止状态之间的典型行为转换。用小波分析方法从海马 LFP 跟踪计算出功...

Watch this Scientific Journal Video about Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat at JoVE.com

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

本研究介绍一种自由运动大鼠脑、心电图、electromyograms 和呼吸信号中局部场电位同时记录的方法。这种技术降低了实验成本, 简化了数据分析, 有助于理解大脑和周围器官之间的相互作用。

自由运动大鼠皮质局部电位、心电图、肌电图和呼吸节律的同步记录

Monitoring the physiological dynamics of the brain and peripheral tissues is necessary for addressing a number of questions about how the brain controls ...

simultaneous-recordings-cortical-local-field-potentials

Research

JoVE Journal

Neuroscience

10.8K 次观看.  The University of Tokyo. 本研究介绍一种自由运动大鼠脑、心电图、electromyograms 和呼吸信号中局部场电位同时记录的方法。这种技术降低了实验成本, 简化了数据分析, 有助于理解大脑和周围器官之间的相互作用。

视频: Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

Simultaneous fMRI and Electrophysiology in the Rodent Brain

To examine the neural basis of the blood oxygenation level dependent (BOLD) magnetic resonance imaging (MRI) signal, we have developed a rodent model in which functional MRI data and in vivo intracortical recording can be performed simultaneously. The combination of MRI and electrical recording is technically challenging because the electrodes used for recording distort the MRI images and the MRI acquisition induces noise in the electrical recording. To minimize the mutual interference of the two modalities, glass microelectrodes were used rather than metal and a noise removal algorithm was implemented for the electrophysiology data. In our studies, two microelectrodes were separately implanted in bilateral primary somatosensory cortices (SI) of the rat and fixed in place. One coronal slice covering the electrode tips was selected for functional MRI. Electrode shafts and fixation positions were not included in the image slice to avoid imaging artifacts. The removed scalp was replaced with toothpaste to reduce susceptibility mismatch and prevent Gibbs ringing artifacts in the images. The artifact structure induced in the electrical recordings by the rapidly-switching magnetic fields during image acquisition was characterized by averaging all cycles of scans for each run. The noise structure during imaging was then subtracted from original recordings. The denoised time courses were then used for further analysis in combination with the fMRI data. As an example, the simultaneous acquisition was used to determine the relationship between spontaneous fMRI BOLD signals and band-limited intracortical electrical activity. Simultaneous fMRI and electrophysiological recording in the rodent will provide a platform for many exciting applications in neuroscience in addition to elucidating the relationship between the fMRI BOLD signal and neuronal activity.

We have developed a method for simultaneous functional magnetic resonance imaging and electrophysiological recording in the rodent brain, providing a platform for the investigation of the relationship between neural activity and the blood oxygenation level dependent (BOLD) MRI signal.

同时在啮齿动物脑功能磁共振成像和电

To examine the neural basis of the blood oxygenation level dependent (BOLD) magnetic resonance imaging (MRI) signal, we have developed a rodent model in ...

科研

神经科学

Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats

Electrocortical responses, elicited by laser heat pulses that selectively activate nociceptive free nerve endings, are widely used in many animal and human studies to investigate the cortical processing of nociceptive information. These laser-evoked brain potentials (LEPs) consist of several transient responses that are time-locked to the onset of laser stimuli. However, the functional properties of the LEP responses are still largely unknown, due to the lack of a sampling technique that can simultaneously record neural activities at the surface of the cortex (i.e., electrocorticogram [ECoG] and scalp electroencephalogram [scalp EEG]) and inside the brain (i.e., local field potential [LFP]). To address this issue, we present here an animal protocol using freely moving rats. This protocol is composed of three main procedures: (1) animal preparation and surgical procedures, (2) a simultaneous recording of ECoG and LFP in response to nociceptive laser stimuli, and (3) data analysis and feature extraction. Specifically, with the help of a 3D-printed protective shell, both ECoG and LFP electrodes implanted on the rat&#39;s skull were securely held together. During data collection, laser pulses were delivered on the rat&#39;s forepaws through gaps in the bottom of the chamber when the animal was in spontaneous stillness. Ongoing white noise was played to avoid the activation of the auditory system by the laser-generated ultrasounds. As a consequence, only nociceptive responses were selectively recorded. Using the standard analytical procedures (e.g., band-pass filtering, epoch extraction, and baseline correction) to extract stimulus-related brain responses, we obtained results showing that LEPs with a high signal-to-noise ratio were simultaneously recorded from ECoG and LFP electrodes. This methodology makes the simultaneous recording of ECoG and LFP activities possible, which provides a bridge of electrocortical signals at the mesoscopic and macroscopic levels, thereby facilitating the investigation of nociceptive information processing in the brain.

We developed a technique that simultaneously records both electrocorticography and local field potentials in response to nociceptive laser stimuli from freely moving rats. This technique helps establish a direct relationship of electrocortical signals at the mesoscopic and macroscopic levels, which facilitates the investigation of nociceptive information processing in the brain.

在自由运动大鼠的反应中, 皮质局部电位和电皮质图谱的同时记录

Electrocortical responses, elicited by laser heat pulses that selectively activate nociceptive free nerve endings, are widely used in many animal and ...

行为学

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

EEG is a useful method to detect electrical activity in the brain. Moreover, it is a widely used diagnostic tool for various neurological conditions, such as epilepsy and neurodegenerative disorders. However, it is technically difficult to obtain EEG recordings in neonates as it requires specialized handling and great care. Here, we present a novel method to record EEG in neonatal rat pups (P8-P15). We designed a simple and reliable electrode using computer pin loci; it can be easily implanted into the skull of a rat pup to record high-quality EEG signals in the normal and epileptic brain. Pups were given an intraperitoneal (i.p.) injection of the neurotoxin kainic acid (KA) to induce epileptic seizures. The surgical implantation performed in this procedure is less expensive than other EEG procedures for neonates. This method allows one to record high-quality and stable EEG signals for more than 1 week. Furthermore, this procedure can also be applied to adult rats and mice to study epilepsy or other neurological disorders.

Here, we introduce a novel technique designed to record electroencephalography (EEG) in freely moving neonatal epileptic pups and describe its procedures, features, and applications. This method allows one to record EEG for more than 1 week.

使用新颖的方法在自由移动的新生大鼠中记录脑电图

EEG is a useful method to detect electrical activity in the brain. Moreover, it is a widely used diagnostic tool for various neurological conditions, such ...

Concurrent Recording of Co-localized Electroencephalography and Local Field Potential in Rodent

Although electroencephalography (EEG) is widely used as a non-invasive technique for recording neural activities of the brain, our understanding of the neurogenesis of EEG is still very limited. Local field potentials (LFPs) recorded via a multi-laminar microelectrode can provide a more detailed account of simultaneous neural activity across different cortical layers in the neocortex, but the technique is invasive. Combining EEG and LFP measurements in a pre-clinical model can greatly enhance understanding of the neural mechanisms involved in the generation of EEG signals, and facilitate the derivation of a more realistic and biologically accurate mathematical model of EEG. A simple procedure for acquiring concurrent and co-localized EEG and multi-laminar LFP signals in the anesthetized rodent is presented here. We also investigated whether EEG signals were significantly affected by a burr hole drilled in the skull for the insertion of a microelectrode. Our results suggest that the burr hole has a negligible impact on EEG recordings.

This protocol describes a simple method for concurrent recording of co-localized electroencephalography (EEG) and multi-laminar local field potential in an anesthetized rat. A burr hole drilled in the skull for the insertion of a microelectrode is shown to produce negligible distortion of the EEG signal.

啮齿动物联合局部脑电图和局部电位的同步记录

Although electroencephalography (EEG) is widely used as a non-invasive technique for recording neural activities of the brain, our understanding of the ...

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats

For studies related to cardiovascular physiology or pathophysiology, blood pressure (BP) and electrocardiography are basic observational parameters. Research focusing on cardiovascular disease models, potential cardiovascular therapeutic targets or pharmaceutical agents requires assessment of systemic arterial pressure and heart rhythm changes. In situations where radio telemetry systems are not available or affordable, the technique of femoral artery cannulation is an alternative way to obtain intra-arterial pressure waveform recordings and systemic BP measurements. This technique is economical and can be performed with standard equipment in animal facilities. However, invasive arterial pressure recording requires cannulation of small arteries, which can be a challenging surgical skill. Here, we present step-by-step protocols for femoral artery cannulation procedures. Key procedures include the calibration of the data acquisition system, tissue dissection and femoral artery cannulation, and setup of the arterial cannulation system for pressure recording. Surface electrocardiography recording procedures are also included. We also present examples of BP recordings from normotensive and hypertensive rats. This protocol allows reliable direct recordings of systemic BP with simultaneous electrocardiography.

Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.

大鼠同时心电图记录及侵入性血压测量

For studies related to cardiovascular physiology or pathophysiology, blood pressure (BP) and electrocardiography are basic observational parameters. ...

医学

小鼠运动皮层中的细胞外电生理记录

Multichannel Extracellular Recording in Freely Moving Mice

The protocol aims to uncover the properties of neuronal firing and network local field potentials (LFPs) in behaving mice carrying out specific tasks by correlating the electrophysiological signals with spontaneous and/or specific behavior. This technique represents a valuable tool in studying the neuronal network activity underlying these behaviors. The article provides a detailed and complete procedure for electrode implantation and consequent extracellular recording in free-moving conscious mice. The study includes a detailed method for implanting the microelectrode arrays, capturing the LFP and neuronal spiking signals in the motor cortex (MC) using a multichannel system, and the subsequent offline data analysis. The advantage of multichannel recording in conscious animals is that a greater number of spiking neurons and neuronal subtypes can be obtained and compared, which allows the evaluation of the relationship between a specific behavior and the associated electrophysiological signals. Notably, the multichannel extracellular recording technique and the data analysis procedure described in the present study can be applied to other brain areas when conducting experiments in behaving mice.

作者聚焦:用于研究自由移动小鼠神经元活动的多通道细胞外记录的进展 

The protocol describes the methodology of extracellular recording in the motor cortex (MC) to reveal extracellular electrophysiological properties in freely moving conscious mice, as well as the data analysis of local field potentials (LFPs) and spikes, which is useful for evaluating the network neural activity underlying behaviors of interest.

自由移动小鼠的多通道细胞外记录

The protocol aims to uncover the properties of neuronal firing and network local field potentials (LFPs) in behaving mice carrying out specific tasks by ...

Multisystem Monitoring of Epileptic Abnormalities in Rabbit: A Method for Simultaneous Video EEG, ECG, Capnography, and Oximetry Recording During Induced Seizures

Source: Bosinski, C., et al. <a href="https://www.jove.com/v/62256">Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits.</a> J. Vis. Exp. (2021).
In this video, we demonstrate a multisystem monitoring method to evaluate neurological and cardiorespiratory abnormalities during intravenous injection of a seizure-inducing drug. The simultaneous monitoring of video EEG, ECG, oximetry, and capnography parameters helps to understand the real-time physiological changes associated with epileptic seizures.

兔癫痫异常的多系统监测:一种在诱导癫痫发作期间同时进行视频 EEG 、 ECG 、 二氧化碳图和血氧饱和度记录的方法

Source: Bosinski, C., et al. Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits. J. Vis. Exp. ...

JoVE Encyclopedia of Experiments

生物学

Large Animal Models

Leporine

In Vivo Electrophysiological Recordings of Brain Activities from Multiple Sites in Rats

Source: Haumesser, J. K., et al. <a href="https://app.jove.com/v/55940">Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats.</a> J. Vis. Exp.(2017).
This video demonstrates the detailed procedure for preparing an anesthetized rat for electrophysiological recordings of brain activity from multiple sites. The scalp is dissected to access the skull, and holes are drilled at precise locations to install and secure electrodes. Tungsten microwire electrodes are then inserted into the targeted location to record the data.

大鼠多个部位大脑活动的体内电生理记录

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

自由运动大鼠皮质局部电位、心电图、肌电图和呼吸节律的同步记录

摘要

探索更多视频

同时在啮齿动物脑功能磁共振成像和电

在自由运动大鼠的反应中, 皮质局部电位和电皮质图谱的同时记录

使用新颖的方法在自由移动的新生大鼠中记录脑电图

啮齿动物联合局部脑电图和局部电位的同步记录

大鼠同时心电图记录及侵入性血压测量

自由移动小鼠的多通道细胞外记录

自由移动小鼠的多通道细胞外记录

自由移动小鼠的多通道细胞外记录

兔癫痫异常的多系统监测:一种在诱导癫痫发作期间同时进行视频 EEG 、 ECG 、二氧化碳图和血氧饱和度记录的方法

大鼠多个部位大脑活动的体内电生理记录

自由运动大鼠皮质局部电位、心电图、肌电图和呼吸节律的同步记录

摘要

探索更多视频

同时在啮齿动物脑功能磁共振成像和电

在自由运动大鼠的反应中, 皮质局部电位和电皮质图谱的同时记录

使用新颖的方法在自由移动的新生大鼠中记录脑电图

啮齿动物联合局部脑电图和局部电位的同步记录

大鼠同时心电图记录及侵入性血压测量

自由移动小鼠的多通道细胞外记录

自由移动小鼠的多通道细胞外记录

自由移动小鼠的多通道细胞外记录

兔癫痫异常的多系统监测:一种在诱导癫痫发作期间同时进行视频 EEG 、 ECG 、 二氧化碳图和血氧饱和度记录的方法

大鼠多个部位大脑活动的体内电生理记录

兔癫痫异常的多系统监测:一种在诱导癫痫发作期间同时进行视频 EEG 、 ECG 、二氧化碳图和血氧饱和度记录的方法