Name: phase contrast magnetic resonance imaging in the rat common carotid artery
Uploaded: 2018-09-05T15:30:00
Description: Watch this Scientific Journal Video about Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery at JoVE.com

This work was supported by grants from Ministry of Science and Technology, Taiwan, under grant number of MOST-105-2314-B-039-044-MY2.

The Institutional Care and Use Committees (IACUC) of the China Medical University approved all procedures.
1. Animal Preparation and Monitoring
<ol>
	<li>Leave all magnetically susceptible objects such as wallets, keys, credit card, etc. outside the scanner room prior to commencing animal preparation for MRI scanning.</li>
	<li>Initially anesthetize the rat (2-month-old male Sprague-Dawley (SD) rat, 280&#8211;350 g) in an induction box using a mixture of 5% isoflurane (ISO) and oxyg...

<ol>
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PC-MRI is a comprehensive approach for non-invasive and longitudinal evaluation of blood flow. We present a protocol for performing PC-MRI of the rat CCA. This procedure is easy to perform in any animal MRI scanner and demonstrates good reproducibility.
The PC-MRI technique has gained increasing popularity in human 10,11 as well as animal studies 4,12. Among these studies, resu...

Magnetic resonance imaging (MRI) is a versatile approach that provides detailed information on internal body structures and physiology, and is increasingly being used for clinical diagnosis and in preclinical animal studies. Animal models are vital for better understanding of considerable clinical implication 1. As animal models considerably differ from humans with respect to anesthesia requirements and physiological parameters, optimization of MRI procedures for such animals assumes importance.
Phase contrast MRI (PC-MRI) is a specialized type of MRI that uses the velocity of flowing spins to quantify flow-related parameters such as blood flow. With PC-MRI, mapping flow patterns in the major arteries using animal models can help shed light on cardiovascular pathologies 2. Moreover, PC-MRI can non-invasively monitor the inherent alternations in blood flow in pathophysiological conditions 3. These observations suggest that PC-MRI is a valuable approach that can be used in animal models of human cardiovascular diseases.
In this report, we describe a method for the quantification of blood flow in the common carotid artery (CCA) of rats. The two CCAs supply the head and neck with oxygenated blood, and the carotid artery disease is a major cause of stroke. Therefore, detecting the early pathology in the CCA is pivotal. This procedure has a duration of approximately 15 min and can be potentially applied to conditions with hemodynamics alterations, such atherosclerosis or stroke.

<table border="0" cellpadding="0" cellspacing="0" width="875">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">7T small animal MRI system</td>
			<td style="width:123px;">Bruker</td>
			<td style="width:344px;"></td>
			<td style="width:193px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Isoflurane&#160;</td>
			<td>Baxter</td>
			<td>1001936040</td>
			<td>anesthetic</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">ECG lead&#160;</td>
			<td>3M</td>
			<td>2269T</td>
			<td style="width:193px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Matlab</td>
			<td>MathWorks</td>
			<td></td>
			<td>sofeware for image processing</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Monitoring and gating system</td>
			<td>SA instruments, Inc</td>
			<td>Model 1030</td>
			<td style="width:193px;"></td>
		</tr>
	</tbody>
</table>

phase contrast magnetic resonance imaging in the rat common carotid artery

Phase contrast magnetic resonance imaging (PC-MRI) is a noninvasive approach that can quantify flow-related parameters such as blood flow. Previous studies have shown that abnormal blood flow may be associated with systemic vascular risk. Thus, PC-MRI can facilitate the translation of data obtained from animal models of cardiovascular diseases to pertinent clinical investigations. In this report, we describe the procedure for measuring blood flow in the common carotid artery (CCA) of rats using cine-gated PC-MRI and discuss relevant analysis methods. This procedure can be performed in a live, anesthetized animal and does not require euthanasia after the procedure. The proposed scanning parameters yield repeatable measurements for blood flow, indicating excellent reproducibility of the results. The PC-MRI procedure described in this article can be used for pharmacological testing, pathophysiological assessment, and cerebral hemodynamics evaluation.

Correct slice geometry is pivotal for ensuring the success of the PC-MRI experiment. The accurate image plane positioning yields a &#34;round&#34; artery shape (Figure 3a), and as angulation increases, i.e., when it is less perpendicular to the artery, the resulting artery geometry becomes ovoid, leading to larger partial volume effects (Figure 3b). Severe partial volume effects would lead to the overestimation of blood ...

Watch this Scientific Journal Video about Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery at JoVE.com

Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging.

Phase contrast magnetic resonance imaging (PC-MRI) is a noninvasive approach that can quantify flow-related parameters such as blood flow. Previous ...

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