Tissue Preparation and Developmental Staging of Drosophila melanogaster Pupa
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Dissection of D. melanogasterAntennal Discs at 0 - 8 h APF
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Dissection of D. melanogaster Mid-pupal Antennae
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Dissection of D. melanogaster Adult Antennae
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Results: Analyses of Prepared Tissues
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Conclusion
副本
The overall goal of staging and dissecting developing pupil and adult periphery olfactory tissues in Drosophila species is to generate samples for molecular and developmental analyses of the peripheral olfactory tissues. This method can help answe
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Here, we present a protocol to stage and dissect developing olfactory tissue from Drosophila species. The dissected tissue can later be used for molecular analyses, such as quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) or RNA sequencing (RNAseq), as well as in vivo analyses such as immunohistochemistry or in situ hybridization.