Results: Representative In Vivo Labeling of Glomerular Cell Surface Proteins
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Conclusion
Transcript
This method can help answer key questions in the nephrology field about the role of cell surface protein trafficking in proteinuric and not proteinuric kidney disease. The main advantages of this technique are that it facilitates the study of cell
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Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.
