Quantification and Normalization of Protein Concentration
2:22
Protein Sample Gel Electrophoresis and Protein Transfer
4:03
Total Protein Staining
5:26
Image Acquisition and Western Blot Analysis
6:39
Results
7:32
Conclusion
副本
This protocol can be used to address important questions in fundamental and clinical research by looking at protein expression across different tissues and time points. To perform reliable quantitative Western blotting, we combine a fluorescent to
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This method describes a robust and reproducible approach for the comparison of protein levels in different tissues and at different developmental timepoints using a standardized quantitative western blotting approach.