Cell Fixation, Immunofluorescence, and Hybridization, and Viral RNA Visualization
4:48
Results: Representative FISH and IF Kaposi s Sarcoma-Associated Herpesvirus (KSHV) Oligonucleotide Analyses
5:52
Conclusion
副本
The protocol is helpful for gaining quantitative insight into the temporal regulation of viral gene products, within the context of host cells, especially in relation to herpes viruses. This technique can help address research questions related to
登录或开始您的免费试用以访问此内容
We describe a protocol utilizing fluorescence in situ hybridization (FISH) to visualize multiple herpesviral RNAs within lytically infected human cells, either in suspension or adherent. This protocol includes quantification of fluorescence producing a nucleocytoplasmic ratio and can be extended for simultaneous visualization of host and viral proteins with immunofluorescence (IF).