Primase DNA Binding Experiment: Blocking Procedure
2:44
Primase DNA Binding Experiment: Protein Binding
3:22
Primase DNA Binding Experiment: Fluorescent Antibody Attachment
5:15
Results: Analysis of High-Throughput Primase Profiling
6:42
Conclusion
Transcript
This protocol describes collection of massive data from protein-DNA binding microarray for primase, an enzyme that transiently binds to specific DNA sequences and in turn catalyzes formation of RNA primers. This video will cover how microarray tec
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Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of enzymes.
