This protocol is significant because the use of frozen tissue simplifies logistics when evaluating multiple tissues, permits sample transfer to a remote laboratory for analysis, and enables deferring a decision to analyze a tissue for months. The
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This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.
