Results: Representative CRISPR/Cas9 Induced Mutations in B. gauderio and B. brachyistius
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Conclusion
副本
CRISPR/Cas9 gene editing in weakly electric fish, particularly in a comparative context, will enable researchers to test hypotheses about how specific genes and genetic variance contribute to phenotypic variation. CRISPR/Cas9 is an efficient metho
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Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae.