Results: Purification and Analysis of KRAS4b Proteins
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Conclusion
副本
Our protocol allows for isolation and separation of properly processed KRAS at high yield. The production of authentically farnesylated and carboxymethylated KRAS makes it possible to perform experiments that have KRAS in their membrane just like
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Prenylation is an important modification on peripheral membrane binding proteins. Insect cells can be manipulated to produce farnesylated and carboxymethylated KRAS4b in quantities that enable biophysical measurements of protein-protein and protein-lipid interactions