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Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

9.3K Views

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10:02 min

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March 31st, 2020

DOI :

10.3791/60797-v

March 31st, 2020

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9,285 Views

AnnJosette Ramirez^1,2, Sophie Astrof¹

¹Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers Biomedical and Health Sciences, ²Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School, Rutgers Biomedical and Health Sciences

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Keywords Visualization

Pharyngeal Arch Arteries

Whole mount Immunohistochemistry

3D Reconstruction

Endothelial Cell Quantification

Aortic Arch Arteries

Congenital Heart Disease

Vessel Formation

Light sheet Microscopy

Permeabilization

Blocking Buffer

Primary Antibody

Secondary Antibody

Plastic Paraffin Molds

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