JoVE Logo
教师资源中心

登录

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

DOI :

10.3791/61541-v

July 21st, 2020

July 21st, 2020

7,235 Views

1Lung and Regenerative Medicine Institutes, Department of Medicine, Cedars-Sinai Medical Center

This article provides a detailed methodology for tissue dissociation and cellular fractionation approaches allowing enrichment of viable epithelial cells from proximal and distal regions of the human lung. Herein these approaches are applied for the functional analysis of lung epithelial progenitor cells through the use of 3D organoids culture models.

Tags

Isolation

-- Views

Related Videos

article

Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

article

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

article

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

article

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

article

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

article

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture

article

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

article

Induction and Analysis of Epithelial to Mesenchymal Transition

article

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

article

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。