Breast Cancer Cell Culture Single Cell Suspension Generation
1:22
Tissue Sample Single Cell Suspension Generation
2:45
Colony Forming Assay
3:54
Mammosphere Assay and 3D Culture Model
5:09
Results: Representative BCSC Identification and Functional Evaluation
6:28
Conclusion
副本
This technique will allow researchers to isolate breast cancer stem cells at maximum purity and viability for the performance of both in vitro and in vivo functional characterization assays. Isolated breast cancer stem cells can also be used to he
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This experimental protocol describes the isolation of BCSCs from breast cancer cell and tissue samples as well as the in vitro and in vivo assays that can be used to assess BCSC phenotype and function.