Characterizing a protein structure is essential for understanding its function. Mass spectrometry has emerged as a powerful tool for this purpose, especially for protein systems that are difficult to study by traditional methods. To study a protei
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The experimental procedures for performing diethylpyrocarbonate-based covalent labeling with mass spectrometric detection are described. Diethylpyrocarbonate is simply mixed with the protein or protein complex of interest, leading to the modification of solvent accessible amino acid residues. The modified residues can be identified after proteolytic digestion and liquid chromatography/mass spectrometry analysis.