Intravenous Injection of Cancer Cells for Metastatic Colony Formation
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Embryo Maintenance During the Metastatic Colony Growth and their Isolation
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Results: Metastatic Colony Isolation for Different Steps of the Screening Protocol
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Conclusion
副本
Our protocol permits an efficient identification of gene or gene combinations that block cancer invasion in viewer. over culture does not require advanced animal facilities for maintenance. As a whole, as a generic or general special libraries can
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This is an effective method to screen for suppressors or drivers of cancer metastasis. Cells, transduced with an expression library, are injected into the chicken chorioallantoic membrane vasculature to form metastatic colonies. Colonies having decreased or increased invasiveness are excised, expanded, reinjected to confirm their phenotype, and finally, analyzed using high throughput sequencing.