Sample Measurement and Actin‐Myosin Contact Establishment
7:37
Results: Position Recording and Force Inversion
8:53
Conclusion
副本
Ultrafast force-clamp spectroscopy is a single molecule technique based on laser tweezers that makes it possible to investigate the chemomechanics of myosins motors under load with the highest time resolution. This technique allows probing very ra
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Presented here is a comprehensive protocol to perform ultrafast force-clamp experiments on processive myosin-5 motors, which could be easily extended to the study of other classes of processive motors. The protocol details all the necessary steps, from the setup of the experimental apparatus to sample preparation, data acquisition and analysis.