The measure of glycogen content in single cells and subcellular organizations is important because in most cell types, single cells adapt differently to changing physiological conditions and diseases. So, the main advantage of this technique is th
登录或开始您的免费试用以访问此内容
A modified post-fixation procedure increases the contrast of glycogen particles in tissue. This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle.