Size Selection of Ribosome Protected Fragments, Identification of P-Sites, and De novo Annotate Translating Open Reading Frames
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Results: Identification of the Novel Open Reding Frames (ORFs) with the Application of RiboCode
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Conclusion
副本
Interpretation of the sequencing data generated by the ribosome profiling experiment is critical for quantitatively measuring the translational activities of ribosomes on mRNA, and for studying the mechanisms of translational regulation. In this p
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Translating ribosomes decode three nucleotides per codon into peptides. Their movement along mRNA, captured by ribosome profiling, produces the footprints exhibiting characteristic triplet periodicity. This protocol describes how to use RiboCode to decipher this prominent feature from ribosome profiling data to identify actively translated open reading frames at the whole-transcriptome level.