Collection of Samples and Total Protein Extraction from Leaf
4:18
Equilibrating the Desalting Column
4:49
Magnetic Beads Washing and Enrichment of Biotinylated Proteins
6:56
Results: AirID-Based Proximity Labeling in Cucumis sativus
7:50
Conclusion
Transcript
AirID is valuable enzyme for protein-protein interaction, and it creates less toxicity and less error-ness in time-taken processes than other proximity-labeling enzymes. A step-by-step protocol is presented for performing the proximity-labeling ex
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Here, we present a step-by-step protocol for performing the proximity labeling (PL) experiment in cucumber (Cucumis sativus L.) using AT4G18020 (APRR2)-AirID protein as a model. The method describes the construction of a vector, the transformation of a construct through agroinfiltration, biotin infiltration, protein extraction, and purification of biotin-labeled proteins through affinity purification technique.
