聚合酶链反应和斑点-印迹杂交用于水样中 钩端螺旋体 检测

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06:05 min

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June 14th, 2024

DOI :

10.3791/65435-v

June 14th, 2024

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1Department of Molecular Biology and Histocompatibility, General Hospital “Dr. Manuel Gea González”, 2Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty, UNAM, 3Department of Ecology of Pathogens, General Hospital “Dr. Manuel Gea González”

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JoVE 208 PCR

此视频中的章节

0:00

Introduction

0:30

Amplification of Leptospira DNA and its Transfer on the Membrane Using Dot‐Blot Apparatus

2:21

DNA•Membrane Fixation and Probe Hybridisation for Leptospira Detection in Water Samples

3:50

Visualization of the DNA Hybrids Using Chemiluminescence for Leptospira Detection in Water Samples

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