Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
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06:05 min
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June 14th, 2024
DOI :
June 14th, 2024
•Eduardo Delgadillo-Tellez1, Beatriz Zavaleta-Villa1, Daniel Atilano-López2, Rigoberto Hernández-Castro3, Angélica Olivo-Díaz1, Erika Margarita Carrillo-Casas1
1Department of Molecular Biology and Histocompatibility, General Hospital “Dr. Manuel Gea González”, 2Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty, UNAM, 3Department of Ecology of Pathogens, General Hospital “Dr. Manuel Gea González”
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Chapters in this video
0:00
Introduction
0:30
Amplification of Leptospira DNA and its Transfer on the Membrane Using Dot‐Blot Apparatus
2:21
DNA•Membrane Fixation and Probe Hybridisation for Leptospira Detection in Water Samples
3:50
Visualization of the DNA Hybrids Using Chemiluminescence for Leptospira Detection in Water Samples
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