针对以下问题发布了勘误表： <a href="https://www.jove.com/t/66075">清醒大鼠锁骨下静脉血液采样</a>。“讨论”部分已更新。
讨论部分的第三段更新自：
在这项研究中，采血失败主要发生在第4天，这可能与反复穿刺导致静脉损伤有关。在第一次采血时，当针刺穿血管时，有明显的穿透感。随着血液样本数量的增加，这种感觉减弱，延长了血液收集时间并增加了失败率。因此，每次采血后，都需要局部压力止血，以促进血管修复，防止局部血肿形成。还建议尝试使用更细的针头，例如胰岛素针头，用于采血。一旦一侧穿刺失败，应按压穿刺部位，让大鼠休息几分钟，然后换到对侧采血。对于药代动力学实验所需的密集血液采样，最好交替从左右锁骨下静脉采集血液。在确实不可用的情况下，可以补充其他采血方法。
自：
在我们的研究中，抽血失败的主要发生发生在第 4 天，这可能与反复穿刺造成的静脉损伤有关。反复穿刺可导致血管壁损伤并引起炎症反应，导致血管壁增厚硬化，甚至诱发血管狭窄。如果穿刺后止血不充分，外渗的血液会进一步引起组织水肿和炎症，从而导致瘢痕组织的形成。这些疤痕组织很难穿透，也会拉扯并导致血管位置移动，所有这些都使血管更难定位和刺穿。在我们的研究中，使用26G注射器（0.45mm）进行采血，相对于人体静脉来说，这很好，但仍然对大鼠静脉造成相当大的损害。在第一次抽血期间，当针头穿过血管时，有明显的穿透感，随着抽血次数的增加，这种穿透感会减少，采血时间更长，失败率更高。因此，我们建议使用较细的胰岛素针头进行采血，采血后应施加足够的压力以防止血肿形成，并应进行交替抽血以充分修复静脉。根据我们的经验，训练有素的抽血师可以在24小时内使用26G针头从同一只大鼠的双侧锁骨下静脉交替抽血8-10次，每次抽血之间平均间隔2-3小时。然而，大鼠可以忍受的最大抽血次数、恢复期和抽血周期可能会受到所用针规、不同实验所需的抽血间隔以及抽血师的熟练程度的影响。这些因素需要在未来的研究中进一步探讨。对于药代动力学实验所需的密集血液采样，最好交替从左右锁骨下静脉采集血液。在确实不可用的情况下，可以补充其他采血方法。

erratum: subclavian vein blood sampling in conscious rats

这更正了文章 <a href="https://dx.doi.org/10.3791/66075">10.3791/66075</a>

针对以下问题发布了勘误表： <a href="https://www.jove.com/t/66075">清醒大鼠锁骨下静脉血液采样</a>。“讨论”部分已更新。

勘误表：有意识大鼠锁骨下静脉血液采样

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erratum-subclavian-vein-blood-sampling-in-conscious-rats

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Erratum: Subclavian Vein Blood Sampling in Conscious Rats

270 次观看.  Lanzhou University Second Hospital. 针对以下问题发布了勘误表： 清醒大鼠锁骨下静脉血液采样。“讨论”部分已更新。

视频: 勘误表：有意识大鼠锁骨下静脉血液采样

<meta charset="utf8"></head><body>改进的血液采样技术以评估小鼠的肠道微生物代谢物

Sequential Blood Collection from Inferior Vena Cava Followed by Portal Vein to Evaluate Gut Microbial Metabolites in Mice

Gut microbial products are known to act both locally within the intestine and get absorbed into circulation, where their effects can extend to numerous distant organ systems. Short-chain fatty acids (SCFA) are one class of metabolites produced by gut microbes during the fermentation of indigestible dietary fiber. They are now recognized&#160;as important contributors to how the gut microbiome influences extra-intestinal organ systems via the gut-lung, gut-brain, and other gut-organ axes throughout the host. SCFAs are absorbed from the colon, through intestinal tissue,&#160;into the portal vein (PV). They then pass through the liver, and are consumed in various organs such as the brain, muscle, adipose tissue, and lungs. SCFAs are most easily measured in the expelled fecal material however, more accurate measurements have been obtained from intra-colonic fecal contents. Here we propose that sampling PV and systemic circulating plasma of a single subject may be preferable for studying the&#160;absorption, transport, and systemic levels of SCFAs in mice. We present a new technique for efficient blood sampling from the PV and inferior vena cava (IVC) that allows for the collection of relatively large volumes of blood from the portal and systemic circulations. This is accomplished by ligating the PV, thereby allowing&#160;for the dilation or enlargement of the PV as it backfills from the mesenteric veins that drain into it. Using this method, we were able to improve the rate of successful collection as well as the total amount of blood collected (up to 0.3 mL from IVC and 0.5 mL from PV).

<meta charset="utf8"></head><body>作者聚焦：通过短链脂肪酸分析实现肠道微生物组-肺组织通讯

The protocol demonstrates a method to collect blood from portal veins and inferior vena cava from mice sequentially to evaluate the production and absorption of gut microbial metabolites.

从下腔静脉连续采集血液，然后进行门静脉采集以评估小鼠的肠道微生物代谢物

Gut microbial products are known to act both locally within the intestine and get absorbed into circulation, where their effects can extend to numerous ...