<meta charset="utf8"></head><body>使用 CMOS-HD-MEA 一致地诱导和记录癫痫样活动

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>2D Workbench</td>
			<td>Cloudray</td>
			<td>LM04CLLD26B</td>
			<td></td>
		</tr>
		<tr>
			<td>4-Aminopyridine</td>
			<td>Sigma-Aldrich</td>
			<td>275875</td>
			<td></td>
		</tr>
		<tr>
			<td>Accura Chip</td>
			<td>3Brain</td>
			<td>Accura HD-MEA</td>
			<td>CMOS-HD-MEA chip</td>
		</tr>
		<tr>
			<td>Agarose</td>
			<td>Thermo Fisher Scientific</td>
			<td>BP160-100</td>
			<td></td>
		</tr>
		<tr>
			<td>Vibration isolation table</td>
			<td>Kinetic Systems</td>
			<td>91010124</td>
			<td></td>
		</tr>
		<tr>
			<td>Beaker for the slice holding chamber, 270 mL</td>
			<td>VWR</td>
			<td>10754-772</td>
			<td></td>
		</tr>
		<tr>
			<td>BioCam</td>
			<td>3Brain</td>
			<td>BioCAM DupleX</td>
			<td>CMOS-HD-MEA platform</td>
		</tr>
		<tr>
			<td>Brainwave Software</td>
			<td>3Brain</td>
			<td>Version 4</td>
			<td>CMOS-HD-MEA software</td>
		</tr>
		<tr>
			<td>Calcium Chloride</td>
			<td>Thermo Fisher Scientific</td>
			<td>BP510-500</td>
			<td></td>
		</tr>
		<tr>
			<td>Carbogen</td>
			<td>Airgas</td>
			<td>X02OX95C2003102</td>
			<td></td>
		</tr>
		<tr>
			<td>Carbogen</td>
			<td>Airgas</td>
			<td>12005</td>
			<td></td>
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		<tr>
			<td>Carbogen Stones</td>
			<td>Supelco</td>
			<td>59277</td>
			<td></td>
		</tr>
		<tr>
			<td>Compresstome</td>
			<td>Precissionary</td>
			<td>VF-300-0Z</td>
			<td></td>
		</tr>
		<tr>
			<td>Computer</td>
			<td>Dell</td>
			<td>Precission3650</td>
			<td></td>
		</tr>
		<tr>
			<td>Crocodile Clip Grounding Cables</td>
			<td>JWQIDI</td>
			<td>B06WGZG17W</td>
			<td></td>
		</tr>
		<tr>
			<td>Detergent</td>
			<td>Metrex</td>
			<td>10-4100-0000</td>
			<td></td>
		</tr>
		<tr>
			<td>D-Glucose</td>
			<td>Macron Fine Chemicals</td>
			<td>4912-12</td>
			<td></td>
		</tr>
		<tr>
			<td>Dihydrogen Sodium Phosphate</td>
			<td>Thermo Fisher Scientific</td>
			<td>BP329-500</td>
			<td></td>
		</tr>
		<tr>
			<td>DinoCam</td>
			<td>Dino-Lite</td>
			<td>AM73915MZTL</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethanol</td>
			<td>Thermo Fisher Scientific</td>
			<td>A407P-4</td>
			<td></td>
		</tr>
		<tr>
			<td>Forceps</td>
			<td>Fine Science Tools</td>
			<td>11980-13</td>
			<td></td>
		</tr>
		<tr>
			<td>Hot plate</td>
			<td>Thermo Fisher Scientific</td>
			<td>SP88857200</td>
			<td></td>
		</tr>
		<tr>
			<td>Ice Machine</td>
			<td>Hoshizaki</td>
			<td>F801MWH</td>
			<td></td>
		</tr>
		<tr>
			<td>Inflow and outflow needles</td>
			<td>Jensen Global</td>
			<td>JG 18-3.0X</td>
			<td></td>
		</tr>
		<tr>
			<td>Inline Solution Heater</td>
			<td>Warner Instruments</td>
			<td>SH-27B</td>
			<td></td>
		</tr>
		<tr>
			<td>Isofluorine</td>
			<td>Dechra</td>
			<td>08PB-STE22002-0122</td>
			<td></td>
		</tr>
		<tr>
			<td>Kim Wipes</td>
			<td>Thermo Fisher Scientific</td>
			<td>06-666</td>
			<td></td>
		</tr>
		<tr>
			<td>Magnesium Chloride</td>
			<td>Thermo Fisher Scientific</td>
			<td>FLM33500</td>
			<td></td>
		</tr>
		<tr>
			<td>Micropipets</td>
			<td>Gilson</td>
			<td>F144069</td>
			<td></td>
		</tr>
		<tr>
			<td>Mili-Q Water Filter</td>
			<td>Mili-Q</td>
			<td>ZR0Q008WW</td>
			<td></td>
		</tr>
		<tr>
			<td>Paintbrush</td>
			<td>Daler Rowney</td>
			<td>AF85 Round: 0</td>
			<td></td>
		</tr>
		<tr>
			<td>Paper Filter</td>
			<td>Whatman</td>
			<td>EW-06648-24</td>
			<td></td>
		</tr>
		<tr>
			<td>Parafilm</td>
			<td>American National Can</td>
			<td>PM996</td>
			<td></td>
		</tr>
		<tr>
			<td>Perfusion System</td>
			<td>Multi Channel System</td>
			<td>PPS2</td>
			<td></td>
		</tr>
		<tr>
			<td>Pipetor</td>
			<td>Thermo Fisher Scientific</td>
			<td>FB14955202</td>
			<td></td>
		</tr>
		<tr>
			<td>Platinum Harp</td>
			<td>3Brain</td>
			<td>3Brain</td>
			<td></td>
		</tr>
		<tr>
			<td>Potassium Chloride</td>
			<td>Thermo Fisher Scientific</td>
			<td>P330-3</td>
			<td></td>
		</tr>
		<tr>
			<td>Razor blade</td>
			<td>Personna</td>
			<td>BP9020</td>
			<td></td>
		</tr>
		<tr>
			<td>Scale</td>
			<td>Metter Toledo</td>
			<td>AB204</td>
			<td></td>
		</tr>
		<tr>
			<td>Scissors</td>
			<td>Solingen</td>
			<td>92008</td>
			<td></td>
		</tr>
		<tr>
			<td>Slice Holding Chamber</td>
			<td>Custom</td>
			<td>Custom</td>
			<td>Custom 3D Printer Design, available upon request</td>
		</tr>
		<tr>
			<td>Sodium Bicarbonate</td>
			<td>Macron Fine Chemicals</td>
			<td>7412-06</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium Chloride</td>
			<td>Thermo Fisher Scientific</td>
			<td>S271-3</td>
			<td></td>
		</tr>
		<tr>
			<td>Temperature Control Box</td>
			<td>Warner Instruments</td>
			<td>TC344B</td>
			<td></td>
		</tr>
		<tr>
			<td>Transfer Pipettes</td>
			<td>Genesee Scientific</td>
			<td>30-200</td>
			<td></td>
		</tr>
		<tr>
			<td>Tubing</td>
			<td>Tygon</td>
			<td>B-44-3 TPE</td>
			<td></td>
		</tr>
		<tr>
			<td>Vibratome VZ-300</td>
			<td>Precissionary</td>
			<td>VF-00-VM-NC</td>
			<td></td>
		</tr>
		<tr>
			<td>Weigh Boat</td>
			<td>Electron Microscopy Sciences</td>
			<td>70040</td>
			<td></td>
		</tr>
	</tbody>
</table>

high-quality seizure-like activity from acute brain slices using a complementary metal-oxide-semiconductor high-density microelectrode array system

Commercially available high-density microelectrode array (HD-MEA) systems, which include an MEA chip with thousands of recording points1,2 and an MEA platform to amplify and digitize the data, are an emerging tool for electrophysiological research. These HD-MEA systems use complementary metal-oxide-semiconductor (CMOS) technology to record electrophysiological data with high sensitivity from cell cultures and ex vivo brain slice preparations. These MEA systems afford unprecedented spatial and temporal resolution to neurophysiological research via high electrode density and quality signal-to-noise ratios3. This technology has mostly been used to study extracellular action potentials, but it can also capture high-quality local field potentials (LFPs) from various neuronal brain slice preparations4,5,6,7,8,9,10,11,12,13,14,15. Due to the above-mentioned high-resolution recording capability of CMOS-HD-MEA systems, users can track electrophysiological activity with great spatial accuracy16,17,18. This capability is particularly relevant to tracking propagation patterns of network LFPs5,12,15,19,20,21. Therefore, CMOS-HD-MEA systems can provide an unprecedented understanding of the propagation patterns of physiological and pathological activity from various cell culture and brain slice preparations. Of particular note, these capabilities of CMOS-HD-MEA systems can allow researchers to contrast seizure patterns of different brain regions simultaneously and assay how various anti-epileptic compounds affect these patterns. By doing so, it provides an innovative method for studying ictogenesis and ictal propagation and for understanding how pharmacology disrupts pathological network activity7,10,14. Therefore, these novel capacities of CMOS-HD-MEA systems can contribute significantly to the research of neurological disorders, as well as aid in drug discovery research5,7,11,22. We aim to provide details on using CMOS-HD-MEA systems to study seizure-like activity.
When using CMOS-HD-MEA systems to study LFPs, such as epileptiform activity in acute brain slices, users can face many challenges, including debilitating electrical noise, keeping the slice healthy during experimentation, and detecting a quality signal from a two-dimensional (2D) CMOS-MEA chip that records only from the surface of the brain slice. This protocol describes basic steps for properly grounding the MEA platform and other equipment used in experimentation, a crucial step that may require individual customization for each lab setup. In addition, we discuss steps to aid in keeping the brain slice healthy during long recordings in the submerged chambers used with CMOS-HD-MEA systems23,24,25. Additionally, in contrast to more common electrophysiological recording methods, which record from deep in the brain slice, most CMOS-HD-MEA systems use 2D chips that do not penetrate into the slice. Therefore, these systems require a healthy neuronal outer layer to produce the majority of the recorded LFP signals. Other challenges include visualizing the massive amount of data generated by thousands of electrodes. To overcome these challenges, we recommend a simple but effective protocol that increases the likelihood of achieving high-quality network epileptiform activity that propagates across the brain slice. We also include a brief description of a publicly available graphic user interface (GUI) we developed with associated resources to aid in data visualization10.
Previous publications have provided related protocols for the use of MEA recording systems26,27,28,29. However, this work aims to assist experimenters using CMOS-HD-MEA systems with 2D chips, specifically those seeking to study high-quality epileptiform activity from brain slices. In addition, we compare two of the most common solution manipulations for induction of seizure-like activity, namely the 0 Mg2+ and 4-AP paradigms, to help users identify the most appropriate convulsant media for their specific application. Although the protocol is focused primarily on the generation of seizure-like activity, it can be modified to explore other electrophysiological phenomena using brain slices.

<meta charset="utf8"></head><body>作者聚焦：使用 CMOS 高密度微电极阵列系统解开癫痫持续状态的癫痫发作动力学和新疗法

使用互补金属氧化物半导体高密度微电极阵列系统从急性脑切片中获得高质量的癫痫样活性

In the Parrish lab, we are keen on understanding how seizures start, propagate, and terminate. We are particularly interested in exploring novel therapies for status epilepticus, a life-threatening condition in which a seizure does not self-terminate. We use CMOS high-density microelectrode array systems in our research.These advanced technologies allow us to record high resolution electrophysiological data from brain slices, capturing detailed local field potentials. This helps us understand complex brain activities, like seizure patterns, with great spatial and temporal precision. In the future, we plan to explore the spatial and temporal propagation patterns of status epilepticus, a prolonged seizure state that often becomes resistant to anti-epileptic medication.We plan to use the information from these studies to find more effective treatments for status epilepticus.

Complementary metal-oxide-semiconductor high-density microelectrode array (CMOS-HD-MEA) systems can record neurophysiological activity from cell cultures ...

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Research

High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

JoVE Journal

Neuroscience

1.1K 次观看.  Brigham Young University. 在 Parrish 实验室，我们热衷于了解癫痫发作是如何开始、传播和终止的。我们对探索癫痫持续状态的新疗法特别感兴趣，癫痫持续状态是一种危及生命的疾病，癫痫发作不会自行终止。我们在研究中使用 CMOS 高密度微电极阵列系统。这些先进的技术使我们能够记录来自脑切片的高分辨率电生理数据，从而捕获详细的局部场电位。这有助于我们以...