<ol>
	<li>Francis, J. A., Bhadeshia, H., Withers, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Welding+residual+stresses+in+ferritic+power+plant+steels.">Welding residual stresses in ferritic power plant steels.</a> Mater Sci Technol. 23 (9), 1009-1020 (2007).</li><li>Withers, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Residual+stress+and+its+role+in+failure.">Residual stress and its role in failure.</a> Rep Prog Phys. 70 (12), 2211 (2007).</li><li>Hornbach, D. J., Preve&#180;y, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+prior+cold+work+on+tensile+residual+stress+development+in+nuclear+weldments.">The effect of prior cold work on tensile residual stress development in nuclear weldments.</a> J. Press Vessel Technol. 124 (3), 359-365 (2002).</li><li>Zondi, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Factors+that+affect+welding-induced+residual+stress+and+distortions+in+pressure+vessel+steels+and+their+mitigation+techniques:+a+review.">Factors that affect welding-induced residual stress and distortions in pressure vessel steels and their mitigation techniques: a review.</a> J Press Vessel Technol. 136 (4), 040801 (2014).</li><li>Ohms, C., Wimpory, R. C., Katsareas, D. E., Youtsos, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=NET+TG1:+Residual+stress+assessment+by+neutron+diffraction+and+finite+element+modeling+on+a+single+bead+weld+on+a+steel+plate.">NET TG1: Residual stress assessment by neutron diffraction and finite element modeling on a single bead weld on a steel plate.</a> Int J Press Vessels Piping. 86 (1), 63-72 (2009).</li><li>Bouchard, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+NeT+bead-on-plate+benchmark+for+weld+residual+stress+simulation.">The NeT bead-on-plate benchmark for weld residual stress simulation.</a> Int J Press Vessel Piping. 86 (1), 31-42 (2009).</li><li>Hofmann, M., Wimpory, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=NET+TG1:+Residual+stress+analysis+on+a+single+bead+weld+on+a+steel+plate+using+neutron+diffraction+at+the+new+engineering+instrument+'STRESS-SPEC'.">NET TG1: Residual stress analysis on a single bead weld on a steel plate using neutron diffraction at the new engineering instrument 'STRESS-SPEC'.</a> Int J Press Vessels Piping. 86 (1), 122-125 (2009).</li><li>Smith, M. C., Smith, A., Ohms, C., Wimpory, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+the+NeT+TG4+international+weld+residual+stress+benchmark.">A review of the NeT TG4 international weld residual stress benchmark.</a> ASME Press Vessels Piping Conf 2015. 6B, (2015).</li><li>Akrivos, V., Smith, M. C., Uddin, M., Brongers, M., Messner, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Material+characterization+on+the+nickel-based+alloy+600/82+NeT-TG6+benchmark+weldments.">Material characterization on the nickel-based alloy 600/82 NeT-TG6 benchmark weldments.</a> Proc ASME Press Vessels Piping Conf 2019. 6B, (2019).</li><li>Wimpory, R. C., Ohms, C., Hofmann, M., Schneider, R., Youtsos, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Statistical+analysis+of+residual+stress+determinations+using+neutron+diffraction.">Statistical analysis of residual stress determinations using neutron diffraction.</a> Int J Press Vessels Piping. 86 (1), 48-62 (2009).</li><li>Ficquet, X., Smith, D. J., Truman, C. E., Kingston, E. J., Dennis, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurement+and+prediction+of+residual+stress+in+a+bead-on-plate+weld+benchmark+specimen.">Measurement and prediction of residual stress in a bead-on-plate weld benchmark specimen.</a> Int J Press Vessels Piping. 86 (1), 20-30 (2009).</li><li>Gilles, P., El-Ahmar, W., Jullien, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Robustness+analyses+of+numerical+simulation+of+fusion+welding+NeT-TG1+application:&amp;#34;Single+weld-bead-on-plate.&amp;#34;.">Robustness analyses of numerical simulation of fusion welding NeT-TG1 application:&amp;#34;Single weld-bead-on-plate.&amp;#34;.</a> Int J Press Vessels Piping. 86 (1), 3-12 (2009).</li><li>Shan, X., Davies, C. M., Wangsdan, T., O'dowd, N. P., Nikbin, K. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thermo-mechanical+modelling+of+a+single-bead-on-plate+weld+using+the+finite+element+method.">Thermo-mechanical modelling of a single-bead-on-plate weld using the finite element method.</a> Int J Press Vessels Piping. 86 (1), 110-121 (2009).</li><li>Bate, S. K., Charles, R., Warren, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Finite+element+analysis+of+a+single+bead-on-plate+specimen+using+SYSWELD.">Finite element analysis of a single bead-on-plate specimen using SYSWELD.</a> Int J Press Vessels Piping. 86 (1), 73-78 (2009).</li><li>Coules, H. E., Smith, D. J., Venkata, K. A., Truman, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+method+for+reconstruction+of+residual+stress+fields+from+measurements+made+in+an+incompatible+region.">A method for reconstruction of residual stress fields from measurements made in an incompatible region.</a> Int J Solids Str. 51 (10), 1980-1990 (2014).</li><li>Smith, M. C., Smith, A. C., Wimpory, R., Ohms, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+the+NeT+Task+Group+1+residual+stress+measurement+and+analysis+round+robin+on+a+single+weld+bead-on-plate+specimen.">A review of the NeT Task Group 1 residual stress measurement and analysis round robin on a single weld bead-on-plate specimen.</a> Int J Press Vessels Piping. 120, 93-140 (2014).</li><li>Na, M. G., Kim, J. W., Lim, D. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prediction+of+residual+stress+for+dissimilar+metals+welding+at+nuclear+power+plants+using+fuzzy+neural+network+models.">Prediction of residual stress for dissimilar metals welding at nuclear power plants using fuzzy neural network models.</a> Nucl Eng Technol. 39 (4), 337-348 (2007).</li><li>Na, M. G., Kim, J. W., Lim, D. H., Kang, Y. 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J., Edwards, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+plasticity+theory+on+predicted+residual+stress+fields+in+numerical+weld+analyses.">The effect of plasticity theory on predicted residual stress fields in numerical weld analyses.</a> Computat Mater Sci. 54, 125-134 (2012).</li><li>Miao, Z., Margetts, L., Vasileiou, A. N., Yin, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surrogate+model+development+using+simulation+data+to+predict+weld+residual+stress:+A+case+study+based+on+the+NeT-TG1+benchmark.">Surrogate model development using simulation data to predict weld residual stress: A case study based on the NeT-TG1 benchmark.</a> Int J Press Vessels Piping. 206, 105014 (2023).</li><li>Seeger, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gaussian+processes+for+machine+learning.">Gaussian processes for machine learning.</a> Int J Neural Sys. 14 (02), 69-106 (2004).</li><li>Chen, S. J., Zhang, Z. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temperature+prediction+of+friction+stir+welding+based+on+Bayesian+neural+network.">Temperature prediction of friction stir welding based on Bayesian neural network.</a> Appl Mech Mater. 48, 1208-1212 (2011).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Abaqus</td>
			<td>Dassault Systems</td>
			<td>2020</td>
			<td></td>
		</tr>
		<tr>
			<td>Intel Fortran Compiler</td>
			<td>Intel Corporation</td>
			<td>17.0.7</td>
			<td></td>
		</tr>
		<tr>
			<td>Keras</td>
			<td>Open Source</td>
			<td>2.6.0</td>
			<td></td>
		</tr>
		<tr>
			<td>Python</td>
			<td>Open Source</td>
			<td>3.8.18</td>
			<td></td>
		</tr>
	</tbody>
</table>

surrogate model development for digital experiments in welding

焊接数字实验的代理模型开发

The manufacturing industry heavily relies on welding processes to join materials, forming integral components across various sectors. Many aspects will ...

surrogate-model-development-for-digital-experiments-in-welding

Research

JoVE Journal

Engineering

Surrogate Model Development for Digital Experiments in Welding

75 次观看.  University of Manchester. 该协议概述了使用通过带有宏函数的 Python 脚本自动生成的数据集构建人工神经网络以进行焊接仿真的一般工作流程。该工作流程根据涉及单道直线焊接的基准案例进行验证。代理模型的预测准确性与有限元仿真具有很强的一致性。

视频: 焊接数字实验的代理模型开发

Real-Time DC-dynamic Biasing Method for Switching Time Improvement in Severely Underdamped Fringing-field Electrostatic MEMS Actuators

Mechanically underdamped electrostatic fringing-field MEMS actuators are well known for their fast switching operation in response to a unit step input bias voltage. However, the tradeoff for the improved switching performance is a relatively long settling time to reach each gap height in response to various applied voltages. Transient applied bias waveforms are employed to facilitate reduced switching times for electrostatic fringing-field MEMS actuators with high mechanical quality factors. Removing the underlying substrate of the fringing-field actuator creates the low mechanical damping environment necessary to effectively test the concept. The removal of the underlying substrate also a has substantial improvement on the reliability performance of the device in regards to failure due to stiction. Although DC-dynamic biasing is useful in improving settling time, the required slew rates for typical MEMS devices may place aggressive requirements on the charge pumps for fully-integrated on-chip designs. Additionally, there may be challenges integrating the substrate removal step into the back-end-of-line commercial CMOS processing steps. Experimental validation of fabricated actuators demonstrates an improvement of 50x in switching time when compared to conventional step biasing results. Compared to theoretical calculations, the experimental results are in good agreement.

The robust device design of fringing-field electrostatic MEMS actuators results in inherently low squeeze-film damping conditions and long settling times when performing switching operations using conventional step biasing. Real-time switching time improvement with DC-dynamic waveforms reduces the settling time of fringing-field MEMS actuators when transitioning between up-to-down and down-to-up states.

实时直流偏置的动态法切换时间改善严重欠阻尼边缘化场静电MEMS致动器

Mechanically underdamped electrostatic fringing-field MEMS actuators are well known for their fast switching operation in response to a unit step input ...

科研

工程学

Ultrasonic Welding of Thermoplastic Composite Coupons for Mechanical Characterization of Welded Joints through Single Lap Shear Testing

This paper presents a novel straightforward method for ultrasonic welding of thermoplastic-composite coupons in optimum processing conditions. The ultrasonic welding process described in this paper is based on three main pillars. Firstly, flat energy directors are used for preferential heat generation at the joining interface during the welding process. A flat energy director is a neat thermoplastic resin film that is placed between the parts to be joined prior to the welding process and heats up preferentially owing to its lower compressive stiffness relative to the composite substrates. Consequently, flat energy directors provide a simple solution that does not require molding of resin protrusions on the surfaces of the composite substrates, as opposed to ultrasonic welding of unreinforced plastics. Secondly, the process data provided by the ultrasonic welder is used to rapidly define the optimum welding parameters for any thermoplastic composite material combination. Thirdly, displacement control is used in the welding process to ensure consistent quality of the welded joints. According to this method, thermoplastic-composite flat coupons are individually welded in a single lap configuration. Mechanical testing of the welded coupons allows determining the apparent lap shear strength of the joints, which is one of the properties most commonly used to quantify the strength of thermoplastic composite welded joints.

A straightforward procedure for ultrasonic welding of thermoplastic composite coupons for basic mechanical testing is described. Key characteristics of this ultrasonic welding process are the use of flat energy directors for simplified process preparation and the use of process data for the fast definition of optimum processing conditions.

通过单搭接剪切试验的热塑性复合材料优惠券焊接接头的力学特性的超声波焊接

This paper presents a novel straightforward method for ultrasonic welding of thermoplastic-composite coupons in optimum processing conditions. The ...

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

A micro-electro-mechanical-system (MEMS) is a widely used component in many industries, including energy, biotechnology, medical, communications, and automotive. However, effective inspection and characterization metrology systems are needed to ensure the functional reliability of MEMS. This study presents a system based on digital holography as a tool for MEMS metrology. Digital holography has gained increasing attention in the past 20 years. With the fast development and decreasing cost of sensor arrays, resolution of such systems has increased broadening potential applications. Thus, it has attracted attention from both research and industry sides as a potential reliable tool for industrial metrology. Indeed, by recording the interference pattern between an object beam (which contains sample height information) and a reference beam on a CCD camera, one can retrieve the quantitative phase information of an object. However, most of digital holographic systems are bulky and thus not easy to implement on industry production lines. The novelty of the system presented is that it is lens-less and thus very compact. In this study, it is shown that the Compact Digital Holographic Microscope (CDHM) can be used to evaluate several characteristics typically consider as criteria in MEMS inspections. The surface profiles of MEMS in both static and dynamic conditions are presented. Comparison with AFM is investigated to validate the accuracy of the CDHM.

We present a compact reflection digital holographic system (CDHM) for inspection and characterization of MEMS devices. A lens-less design using a diverging input wave providing natural geometrical magnification is demonstrated. Both static and dynamic studies are presented.

紧凑的镜头少数字全息显微镜的MEMS检测与表征

A micro-electro-mechanical-system (MEMS) is a widely used component in many industries, including energy, biotechnology, medical, communications, and ...

Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells

Silicon solar cell manufacturing is an expensive and high energy consuming process. In contrast, dye sensitized solar cell production is less environmentally damaging with lower processing temperatures presenting a viable and low cost alternative to conventional production. This paper further enhances these environmental credentials by evaluating the digital printing and therefore additive production route for these cells. This is achieved here by investigating the formation and performance of a metal oxide photoelectrode using nanoparticle sized titanium dioxide. An ink-jettable material was formulated, characterized and printed with a piezoelectric inkjet head to produce a 2.6 &#181;m thick layer. The resultant printed layer was fabricated into a functioning cell with an active area of 0.25 cm2 and a power conversion efficiency of 3.5%. The binder-free formulation resulted in a reduced processing temperature of 250 &#176;C, compatible with flexible polyamide substrates which are stable up to temperatures of 350 &#730;C. The authors are continuing to develop this process route by investigating inkjet printing of other layers within dye sensitized solar cells.

This paper investigates the suitability of inkjet printing for the manufacturing of dye-sensitized solar cells. A binder-free TiO2 nanoparticle ink was formulated and printed onto a FTO glass substrate. The printed layer was fabricated into a cell with an active area of 0.25 cm2 and an efficiency of 3.5%.

钛白粉用于染料敏化太阳能电池的数码印刷

Silicon solar cell manufacturing is an expensive and high energy consuming process. In contrast, dye sensitized solar cell production is less ...

Combustion Characterization and Model Fuel Development for Micro-tubular Flame-assisted Fuel Cells

Combustion based power generation has been accomplished for many years through a number of heat engine systems. Recently, a move towards small scale power generation and micro combustion as well as development in fuel cell research has created new means of power generation that combine solid oxide fuel cells with open flames and combustion exhaust. Instead of relying upon the heat of combustion, these solid oxide fuel cell systems rely on reforming of the fuel via combustion to generate syngas for electrochemical power generation. Procedures were developed to assess the combustion by-products under a wide range of conditions. While theoretical and computational procedures have been developed for assessing fuel-rich combustion exhaust in these applications, experimental techniques have also emerged. The experimental procedures often rely upon a gas chromatograph or mass spectrometer analysis of the flame and exhaust to assess the combustion process as a fuel reformer and means of heat generation. The experimental techniques developed in these areas have been applied anew for the development of the micro-tubular flame-assisted fuel cell. The protocol discussed in this work builds on past techniques to specify a procedure for characterizing fuel-rich combustion exhaust and developing a model fuel-rich combustion exhaust for use in flame-assisted fuel cell testing. The development of the procedure and its applications and limitations are discussed.

A protocol for creating a model fuel-rich combustion exhaust is developed through combustion characterization and is applied for micro-tubular flame-assisted fuel cell testing and research.

燃烧特性参数和模型燃料开发的微型管状火焰辅助燃料电池

Combustion based power generation has been accomplished for many years through a number of heat engine systems. Recently, a move towards small scale power ...

Generating Lap Joints Via Friction Stir Spot Welding on DP780 Steel

Friction stir spot welding (FSSW), a derivative of friction stir welding (FSW), is a solid-state welding technique that was developed in 1991. An industry application was found in the automotive industry in 2003 for the aluminum alloy that was used in the rear doors of automobiles. Friction stir spot welding is mostly used in Al alloys to create lap joints. The benefits of friction stir spot welding include a nearly 80% melting temperature that lowers the thermal deformation welds without splashing compared to resistance spot welding. Friction stir spot welding includes 3 steps: plunging, stirring, and retraction. In the present study, other materials including high strength steel are also used in the friction stir welding method to create joints. DP780, whose traditional welding process involves the use of resistance spot welding, is one of several high strength steel materials used in the automotive industry. In this paper, DP780 was used for friction stir spot welding, and its microstructure and microhardness were measured. The microstructure data showed that there was a fusion zone with fine grain and a heat effect zone with island martensite. The microhardness results indicated that the center zone exhibited a greater degree of hardness compared with the base metal. All data indicated that the friction stir spot welding used in dual phase steel 780 can create a good lap joint. In the future, friction stir spot welding can be used in high-strength steel welding applied in industrial manufacturing processes.

Here, we present a friction stir spot welding (FSSW) protocol on dual phase 780 steel. A tool pin with high-speed rotation generates heat from friction to soften the material, and then, the pin plunges into 2 sheet joints to create the lap joint.

通过 DP780 钢上的摩擦搅拌点焊生成圈接头

Friction stir spot welding (FSSW), a derivative of friction stir welding (FSW), is a solid-state welding technique that was developed in 1991. An industry ...

Crack Monitoring in Resonance Fatigue Testing of Welded Specimens Using Digital Image Correlation

A procedure using digital image correlation (DIC) to detect cracks on welded specimens during fatigue tests on resonance testing machines is presented. It is intended as a practical and reproducible procedure to identify macroscopic cracks at an early stage and monitor crack propagation during fatigue tests. It consists of strain field measurements at the weld using DIC. Images are taken at fixed load cycle intervals. Cracks become visible in the computed strain field as elevated strains. This way, the whole width of a small-scale specimen can be monitored to detect where and when a crack initiates. Subsequently, it is possible to monitor the development of the crack length. Because the resulting images are saved, the results are verifiable and comparable. The procedure is limited to cracks initiating at the surface and is intended for fatigue tests under laboratory conditions. By visualizing the crack, the presented procedure allows direct observation of macrocracks from their formation until rupture of the specimen.

Digital image correlation is used in fatigue tests on a resonance testing machine to detect macroscopic cracks and monitor crack propagation in welded specimens. Cracks on the specimen surface become visible as increased strains.

使用数字图像相关性对焊接试样共振疲劳测试中的裂纹监测

A procedure using digital image correlation (DIC) to detect cracks on welded specimens during fatigue tests on resonance testing machines is presented. It ...

Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments

Described is an experimental procedure that enables high-power laser irradiation of microfabricated targets. Targets are brought to the laser focus by a closed feedback loop that operates between the target manipulator and a ranging sensor. The target fabrication process is explained in detail. Representative results of MeV-level proton beams generated by irradiation of 600 nm thick gold foils at a rate of 0.2 Hz are given. The method is compared with other replenishable target systems and the prospects of increasing the shot rates to above 10 Hz are discussed.

A protocol is presented for automated irradiation of thin gold foils with high intensity laser pulses. The protocol includes a step-by-step description of the micromachining target fabrication process and a detailed guide for how targets are brought to the laser&#39;s focus at a rate of 0.2 Hz.

用于密集激光辐照实验的微制造目标的自动交付

Described is an experimental procedure that enables high-power laser irradiation of microfabricated targets. Targets are brought to the laser focus by a ...

A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education

This paper describes an educational kit based on digital microfluidics. A protocol for luminol-based chemiluminescence experiment is reported as a specific example. It also has fluorescent imaging capability and closed humidified enclosure based on an ultrasonic atomizer to prevent evaporation. The kit can be assembled within a short period of time and with minimal training in electronics and soldering. The kit allows both undergraduate/graduate students and enthusiasts to obtain hands-on experience in microfluidics in an intuitive way and be trained to gain familiarity with digital microfluidics.

We describe an educational kit that allows users to execute multiple experiments and gain hands-on experience on digital microfluidics.

基于数字微流体的多功能套件，用于科学教育

This paper describes an educational kit based on digital microfluidics. A protocol for luminol-based chemiluminescence experiment is reported as a ...

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress

The proper use of statistical modeling in NGS data analysis requires an advanced level of expertise. There has recently been a growing consensus on using generalized linear models for differential analysis of RNA-Seq data and the advantage of mixture models to perform co-expression analysis. To offer a managed setting to use these modeling approaches, we developed DiCoExpress that provides a standardized R pipeline to perform an RNA-Seq analysis. Without any particular knowledge in statistics or R programming, beginners can perform a complete RNA-Seq analysis from quality controls to co-expression through differential analysis based on contrasts inside a generalized linear model. An enrichment analysis is proposed both on the lists of differentially expressed genes, and the co-expressed gene clusters. This video tutorial is conceived as a step-by-step protocol to help users take full advantage of DiCoExpress and its potential in empowering the biological interpretation of an RNA-Seq experiment.

DiCoExpress is a script-based tool implemented in R to perform an RNA-Seq analysis from quality control to co-expression. DiCoExpress handles complete and unbalanced design up to 2 biological factors. This video tutorial guides the user through the different features of DiCoExpress.

使用双快通分析多因子RNA-Seq实验

The proper use of statistical modeling in NGS data analysis requires an advanced level of expertise. There has recently been a growing consensus on using ...