Immunoprobing Membrane with Directly Conjugated Secondary Antibody
10:45
Immunoprobing with Avidin-Biotin Coupling to Secondary Antibody
14:26
Visualization of Immunoblot with Chromogenic Substrates
15:32
Conclusion
Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.
