Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

DOI :

10.3791/789-v

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June 27th, 2008

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14,387 Views

Kelly Morgan¹, Michael Kharas¹, Elaine Dzierzak², D. Gary Gilliland^1,3

¹Department of Hematology and Oncology, Brigham and Women's Hospital and Harvard Medical School, ²Department of Cell Biology and Genetics, Erasmus University Medical Center, ³Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital and Harvard Medical School

Chapters

This video shows how to micro-dissect the yolk sac and aorta-gonad-mesonephros region from embryos and use flow cytometry to sort hematopoietic stem cells.

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