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Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

14.5K Views

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10:38 min

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June 27th, 2008

DOI :

10.3791/789-v

June 27th, 2008

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1Department of Hematology and Oncology, Brigham and Women's Hospital and Harvard Medical School, 2Department of Cell Biology and Genetics, Erasmus University Medical Center, 3Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital and Harvard Medical School

Explore More Videos

Isolation
Early Hematopoietic Stem Cells
Murine Yolk Sac
AGM
Mouse Embryo
Blood System
Fetal Liver
Bone Marrow
Microdissection
Flow Cytometric Sorting
Pure Population
Stem Cell Pool
Functional Differences
Genetic Models
Video Demonstration
FACS Plot
Functional Assays
Colony Assays
Bone Marrow Transplants

Chapters in this video

0:00

Title

0:51

Introduction

2:04

Dissection and Isolation of Early Hematopoietic Stem Cells

7:19

Dissociation and FACS Analysis

9:57

Conclusion

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