in-vitro-pancreas-organogenesis-from-dispersed-mouse-embryonic

<em>In Vitro</em> Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

The pancreas is an essential organ that regulates glucose homeostasis and secretes digestive enzymes. Research on pancreas embryogenesis has led to the ...

Swiss Institute for Experimental Cancer Research

Chest infections in young neuromuscular patients: the critical importance of preserving Vital Capacity.

Expert management of tracheal intubation has become fundamental to the routine practice of pulmonary physicians who work in respiratory intensive care units (ICUs). In Italy, tracheal intubation is not included as part of the training in respiratory medicine, and pulmonary physicians are usually dissuaded from managing intubations.

Management of tracheal intubation in the respiratory intensive care unit by pulmonary physicians.

In the context of a cellular therapy for diabetes, methods for pancreatic progenitor expansion and subsequent differentiation into insulin-producing beta cells would be extremely valuable. Here we establish three-dimensional culture conditions in Matrigel that enable the efficient expansion of dissociated mouse embryonic pancreatic progenitors. By manipulating the medium composition we generate either hollow spheres, which are mainly composed of pancreatic progenitors, or complex organoids that spontaneously undergo pancreatic morphogenesis and differentiation. The in vitro maintenance and expansion of pancreatic progenitors require active Notch and FGF signaling, thus recapitulating in vivo niche signaling interactions. Our experiments reveal new aspects of pancreas development, such as a community effect by which small groups of cells better maintain progenitor properties and expand more efficiently than isolated cells, as well as the requirement for three-dimensionality. Finally, growth conditions in chemically defined biomaterials pave the way for testing the biophysical and biochemical properties of the niche that sustains pancreatic progenitors.

Artificial three-dimensional niches deconstruct pancreas development in vitro.

Sarcopenia is thought to be associated with mitochondrial (Mito) loss. It is unclear whether the decrease in Mito content is consequent to aging per se or to decreased physical activity.

Chiara Greggio

Ecole Polytechnique Fédérale de Lausanne,
School of Life Sciences,
Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences

Swiss Institute for Experimental Cancer Research

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

Chiara Greggio

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Swiss Institute for Experimental Cancer Research

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

Ecole Polytechnique Fédérale de Lausanne,
School of Life Sciences,
Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences