quantitative-analysis-alternative-pre-mrna-splicing-mouse-brain

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA <em>In Situ</em> Hybridization Assay

Alternative splicing (AS) occurs in more than 90% of human genes. The expression pattern of an alternatively spliced exon is often regulated in a cell ...

Dongfang Hospital of Beijing University of Chinese Medicine

Metabolic syndrome (MS) and type 2 diabetes mellitus (T2DM) have been associated with insulin-resistance; however, the effective therapies in improving insulin sensitivity are limited. This study is aimed at investigating the effect of Guava Leaf (GL) extracts on glucose tolerance and insulin resistance in SHRSP.Z-Leprfa/Izm rats (SHRSP/ZF), a model of spontaneously metabolic syndrome.

Guava leaf extracts promote glucose metabolism in SHRSP.Z-Leprfa/Izm rats by improving insulin resistance in skeletal muscle.

Isoquercitrin, a flavonoid compound that is widely distributed in medicinal and dietary plants, possesses many biological activities, including inhibition of adipocyte differentiation. In this study, we investigated the effect of isoquercitrin on lipid accumulation and its molecular mechanisms in rat hepatoma H4IIE cells.

Isoquercitrin activates the AMP-activated protein kinase (AMPK) signal pathway in rat H4IIE cells.

Chinese medicine comprised of all natural herbs is widespread used in the treatment of diabetic nephropathy (DN). Podocyte contributes to the integrity of glomerular filtration barrier whose injury plays an important role in the initiation and progression of DN. Our study aimed to investigate the effect of Qiwei granules on podocyte lesion in diabetic KK-A(y) mice kidney and its underlying mechanism.

Qiwei granules alleviates podocyte lesion in kidney of diabetic KK-Ay mice.

Asiatic acid (AA) has been demonstrated to exhibit anti-diabetic activity. However, the mechanisms and underlying signaling pathways remain to be elucidated. The present study was performed to confirm the protective effect of AA and demonstrate its ability to regulate the phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (AKT)/glycogen synthase kinase‑3β (GSK‑3β) signaling pathway in db/db mice. Db/db mice fed on a high‑fat diet were used to model diabetes mellitus. Modeled mice were divided randomly into the model control, pioglitazone hydrochloride tablet (PH) and AA groups. Age‑matched C57 BL/6J mice served as normal controls. Lipid and glucose levels, and glycogen synthesis rates were assessed following treatment. Pathological changes were detected using hematoxylin and eosin staining. Expression of the PI3K/AKT/GSK‑3β signaling pathway at the mRNA level was measured using quantitative polymerase chain reaction analysis. The model control group revealed typical characteristics of obesity and diabetes, including high glucose and lipid levels, and decreased glycogen synthesis. Four weeks of treatment with AA or PH ameliorated these abnormalities. AA and PH treatments mitigated the upregulation of PI3K, AKT, insulin receptor, and insulin receptor substrate‑1 mRNA expression in modeled mice. Furthermore, AA and PH treatments decreased GSK‑3β and glucose‑6‑phosphatase mRNA expression compared with the normal control group. The results of the present study confirmed that AA possesses anti‑diabetic activity in db/db mice. The PI3K/AKT/GSK‑3β signaling pathway may mediate this protective effect.

Protective effects of asiatic acid in a spontaneous type 2 diabetic mouse model.

This study presents a systematic meta-analysis of the correlation between  () infection and autoimmune thyroid diseases (AITD).

Tonghua Liu

Department of Endocrinology

Dongfang Hospital of Beijing University of Chinese Medicine

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay