rna-based-reprogramming-human-primary-fibroblasts-into-induced

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Induced pluripotent stem cells (iPSCs) have proven to be a valuable tool to study human development and disease. Further advancing iPSCs as a regenerative ...

circle-seq-for-interrogation-of-off-target-gene-editing

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Circularization for In Vitro Reporting of Cleavage Effects by Sequencing (CIRCLE-seq) is a novel technique developed for the impartial identification of ...

University of Colorado School of Medicine, Anschutz Medical Campus

Endothelial cells (ECs) are important components of the circulatory system. These cells can be used for in vitro modeling of cardiovascular diseases and in regenerative medicine to promote vascularization of engineered tissue constructs. However, low proliferative capacity and patient-to-patient variability limit the use of primary ECs in the clinic and disease modeling. ECs differentiated from human induced pluripotent stem cells (iPSCs) can serve as a viable alternative to primary ECs for these applications. This is because human iPSCs can proliferate indefinitely and have the potential to differentiate into a variety of somatic cell lines, providing a renewable source of patient-specific cells. Here, we present an optimized, highly reproducible method for the differentiation of human iPSCs toward vascular ECs. The protocol relies on the activation of the WNT signaling pathway and the use of growth factors and small molecules. The resulting iPSC-derived ECs can be cultured for multiple passages without losing their functionality and are suitable for both in vitro and in vivo studies.

Igor Kogut

Department of Dermatology,
Charles C. Gates Center for Regenerative Medicine,
Stem Cell Biobank and Disease Modeling Core,
Gates Institute

University of Colorado School of Medicine, Anschutz Medical Campus

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Igor Kogut

Department of Dermatology,Charles C. Gates Center for Regenerative Medicine,Stem Cell Biobank and Disease Modeling Core,Gates Institute

University of Colorado School of Medicine, Anschutz Medical Campus

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Department of Dermatology,
Charles C. Gates Center for Regenerative Medicine,
Stem Cell Biobank and Disease Modeling Core,
Gates Institute