super-resolution-microscopy-synaptonemal-complex-within

Super-Resolution Microscopy of the Synaptonemal Complex Within the <em>Caenorhabditis elegans</em> Germline

During meiosis, homologous chromosomes must recognize and adhere to one another to allow for their correct segregation. One of the key events that secures ...

European Molecular Biology Laboratory

Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.

Rory M. Power

EMBL Imaging Centre

European Molecular Biology Laboratory

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Rory M. Power

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European Molecular Biology Laboratory

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

EMBL Imaging Centre