Brian T. Chait

SYT associates with human SNF/SWI complexes and the C-terminal region of its fusion partner SSX1 targets histones.

N-terminal contributions of the gamma-subunit of fetal hemoglobin to its tetramer strength: remote effects at subunit contacts.

X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity.

Mass spectrometric analysis of the N terminus of translational initiation factor eIF4G-1 reveals novel isoforms.

On the nature of the chemical noise in MALDI mass spectra.

The N-CoR-HDAC3 nuclear receptor corepressor complex inhibits the JNK pathway through the integral subunit GPS2.

Requirement of Ca2+ and CaMKII for Stat1 Ser-727 phosphorylation in response to IFN-gamma.

Crystal structure and mechanism of a calcium-gated potassium channel.

The open pore conformation of potassium channels.

Tyrosine sulfation of CCR5 N-terminal peptide by tyrosylprotein sulfotransferases 1 and 2 follows a discrete pattern and temporal sequence.

Proteomic analysis of the mammalian nuclear pore complex.

ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination.

Ezh2 controls B cell development through histone H3 methylation and Igh rearrangement.

CIAP1 and the serine protease HTRA2 are involved in a novel p53-dependent apoptosis pathway in mammals.

Detection of secreted peptides by using hypothesis-driven multistage mass spectrometry.

A modular cross-linking approach for exploring protein interactions.

X-ray structure of a voltage-dependent K+ channel.

Localization of the PE methylation pathway and SR-BI to the canalicular membrane: evidence for apical PC biosynthesis that may promote biliary excretion of phospholipid and cholesterol.

Dynorphin A(1-17) biotransformation in striatum of freely moving rats using microdialysis and matrix-assisted laser desorption/ionization mass spectrometry.

"De novo" peptide sequencing by MALDI-quadrupole-ion trap mass spectrometry: a preliminary study.

Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit.

Structure of microcin J25, a peptide inhibitor of bacterial RNA polymerase, is a lassoed tail.

Biophysical methods: doing more with less.

Virtual gating and nuclear transport: the hole picture.

Improved beta-elimination-based affinity purification strategy for enrichment of phosphopeptides.

Targeted proteomic study of the cyclin-Cdk module.

POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex.

Analysis of protein phosphorylation by hypothesis-driven multiple-stage mass spectrometry.

TIN2 binds TRF1 and TRF2 simultaneously and stabilizes the TRF2 complex on telomeres.

E protein silencing by the leukemogenic AML1-ETO fusion protein.

Dual functions, clamp opening and primer-template recognition, define a key clamp loader subunit.

Characterization of karyopherin cargoes reveals unique mechanisms of Kap121p-mediated nuclear import.

Domain-specific incorporation of noninvasive optical probes into recombinant proteins.

Components of coated vesicles and nuclear pore complexes share a common molecular architecture.

Preferential phosphorylation of R-domain Serine 768 dampens activation of CFTR channels by PKA.

Channel function is dissociated from the intrinsic kinase activity and autophosphorylation of TRPM7/ChaK1.

Proteomic and genomic characterization of chromatin complexes at a boundary.

N-terminal acetylation and protonation of individual hemoglobin subunits: position-dependent effects on tetramer strength and cooperativity.

Polyglutamine-expanded ataxin-7 inhibits STAGA histone acetyltransferase activity to produce retinal degeneration.

Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.

MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription.

The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly.

Long range allosteric control of cytoplasmic dynein ATPase activity by the stalk and C-terminal domains.

Fluorescent proteins as proteomic probes.

I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions.

A method for the rapid and efficient elution of native affinity-purified protein A tagged complexes.

Quantitative analysis of protein phosphorylation in mouse brain by hypothesis-driven multistage mass spectrometry.

The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control.

Linker chains of the gigantic hemoglobin of the earthworm Lumbricus terrestris: primary structures of linkers L2, L3, and L4 and analysis of the connectivity of the disulfide bonds in linker L1.

Simple fold composition and modular architecture of the nuclear pore complex.

Protease accessibility laddering: a proteomic tool for probing protein structure.

Synergistic functions of SII and p300 in productive activator-dependent transcription of chromatin templates.

Regulation of hypermutation by activation-induced cytidine deaminase phosphorylation.

A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling.

Induction of autophagy in axonal dystrophy and degeneration.

Tracking and elucidating alphavirus-host protein interactions.

Chemistry. Mass spectrometry: bottom-up or top-down?

Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo.

Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs.

Chromosomal enrichment and activation of the aurora B pathway are coupled to spatially regulate spindle assembly.

Long-distance combinatorial linkage between methylation and acetylation on histone H3 N termini.

Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex.

Efficiency, selectivity, and robustness of nucleocytoplasmic transport.

Human embryonic, fetal, and adult hemoglobins have different subunit interface strengths. Correlation with lifespan in the red cell.

Prediction of cyclin-dependent kinase phosphorylation substrates.

Crystal structure of a Kir3.1-prokaryotic Kir channel chimera.

Methylation of a histone mimic within the histone methyltransferase G9a regulates protein complex assembly.

Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration.

Comprehensive analysis of diverse ribonucleoprotein complexes.

Multifunctional class I transcription in Trypanosoma brucei depends on a novel protein complex.

Determining the architectures of macromolecular assemblies.

The molecular architecture of the nuclear pore complex.

Affinity isolation and I-DIRT mass spectrometric analysis of the Escherichia coli O157:H7 Sakai RNA polymerase complex.

Limited effects of beta-endorphin compared to loperamide or fentanyl in a neuroendocrine biomarker assay in non-human primates.

Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions.

Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage.

Efficient identification of phosphorylation by mass spectrometric phosphopeptide fingerprinting.

A novel high-capacity ion trap-quadrupole tandem mass spectrometer.

Human cytomegalovirus protein UL38 inhibits host cell stress responses by antagonizing the tuberous sclerosis protein complex.

Ku80 脱从 DNA 双链断裂致泛素化。

Sequential tyrosine sulfation of CXCR4 by tyrosylprotein sulfotransferases.

Regulation of class switch recombination and somatic mutation by AID phosphorylation.

High-yield isolation and subcellular proteomic characterization of nuclear and subnuclear structures from trypanosomes.

Artificial nanopores that mimic the transport selectivity of the nuclear pore complex.

Carbon dioxide-induced anesthesia results in a rapid increase in plasma levels of vasopressin.

Using genetic mouse models to study the biology and pathology of autophagy in the central nervous system.

Distinct regulation of autophagic activity by Atg14L and Rubicon associated with Beclin 1-phosphatidylinositol-3-kinase complex.

Rapid sensitive analysis of cysteine rich peptide venom components.

Proteomic studies of a single CNS synapse type: the parallel fiber/purkinje cell synapse.

Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor.

Identification of SMARCAL1 as a component of the DNA damage response.

Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates.

Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA.

GINS motion reveals replication fork progression is remarkably uniform throughout the yeast genome.

Host factors associated with the Sindbis virus RNA-dependent RNA polymerase: role for G3BP1 and G3BP2 in virus replication.

Human cytomegalovirus pUL83 stimulates activity of the viral immediate-early promoter through its interaction with the cellular IFI16 protein.

Enhancement of transport selectivity through nano-channels by non-specific competition.

Developmental expression of human hemoglobins mediated by maturation of their subunit interfaces.

Human cytomegalovirus UL29/28 protein interacts with components of the NuRD complex which promote accumulation of immediate-early RNA.

Protein quantitation using mass spectrometry.

Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction.

Temporal regulation of gene expression of the Thermus thermophilus bacteriophage P23-45.

Global analysis of Cdc14 phosphatase reveals diverse roles in mitotic processes.

Amino-terminal phosphorylation of activation-induced cytidine deaminase suppresses c-myc/IgH translocation.

Phosphorylation-dependent 14-3-3 binding to LRRK2 is impaired by common mutations of familial Parkinson's disease.

A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae.

High-Capacity Ion Trap Coupled to a Time-of-Flight Mass Spectrometer for Comprehensive Linked Scans with no Scanning Losses.

Affinity purification of protein complexes.

Conjugation of magnetic beads for immunopurification of protein complexes.

Mass spectrometry in the postgenomic era.

Sequence and structural convergence of broad and potent HIV antibodies that mimic CD4 binding.

Cdk1 uncouples CtIP-dependent resection and Rad51 filament formation during M-phase double-strand break repair.

Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs.

Intrinsic regulation of hemoglobin expression by variable subunit interface strengths.

Quantitative Chemical Proteomics Approach To Identify Post-translational Modification-Mediated Protein-Protein Interactions.

Smyd2 controls cytoplasmic lysine methylation of Hsp90 and myofilament organization.

Transcriptional regulation by Pol II(G) involving mediator and competitive interactions of Gdown1 and TFIIF with Pol II.

Proteomics on the rims: insights into the biology of the nuclear envelope and flagellar pocket of trypanosomes.

Structure-function mapping of a heptameric module in the nuclear pore complex.

RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3')pp(5')G intermediates.

Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.

Constrained de novo sequencing of conotoxins.

The biochemical anatomy of cortical inhibitory synapses.

Strategy for identifying dendritic cell-processed CD4+ T cell epitopes from the HIV gag p24 protein.

Activation of DSB processing requires phosphorylation of CtIP by ATR.

Examining post-translational modification-mediated protein-protein interactions using a chemical proteomics approach.

Rif1 prevents resection of DNA breaks and promotes immunoglobulin class switching.

SMARCA3, a chromatin-remodeling factor, is required for p11-dependent antidepressant action.

The yeast Snt2 protein coordinates the transcriptional response to hydrogen peroxide-mediated oxidative stress.

Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly.

Supervillin binding to myosin II and synergism with anillin are required for cytokinesis.

The nuclear basket proteins Mlp1p and Mlp2p are part of a dynamic interactome including Esc1p and the proteasome.

Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition.

Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity.

Sample limited characterization of a novel disulfide-rich venom peptide toxin from terebrid marine snail Terebra variegata.

NPC mimics: probing the mechanism of nucleocytoplasmic transport.

Method for identifying phosphorylated substrates of specific cyclin/cyclin-dependent kinase complexes.

Molecular architecture and function of the SEA complex, a modulator of the TORC1 pathway.

Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels.

Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex.

Structural characterization by cross-linking reveals the detailed architecture of a coatomer-related heptameric module from the nuclear pore complex.

A pipeline for determining protein-protein interactions and proximities in the cellular milieu.

A robust pipeline for rapid production of versatile nanobody repertoires.

Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.

Touching from a distance.

Maximizing ion transmission from atmospheric pressure into the vacuum of mass spectrometers with a novel electrospray interface.

Protein kinase A directly phosphorylates metabotropic glutamate receptor 5 to modulate its function.

Optimizing Electrospray Interfaces Using Slowly Diverging Conical Duct (ConDuct) Electrodes.

Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives.