Frank Buchholz

Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.

Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA.

Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation.

RNA interference: gene silencing in the fast lane.

Alternative approaches for efficient inhibition of hepatitis C virus RNA replication by small interfering RNAs.

Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation and whole embryo culture.

DEQOR: a web-based tool for the design and quality control of siRNAs.

An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division.

RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells.

Functional genomic analysis of cell division by endoribonuclease-prepared siRNAs.

Selective expression of the Cre recombinase in late-stage thymocytes using the distal promoter of the Lck gene.

Production of endoribonuclease-prepared short interfering RNAs for gene silencing in mammalian cells.

Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.

Enzymatically prepared RNAi libraries.

Minimizing the risk of reporting false positives in large-scale RNAi screens.

RNAi in embryonic stem cells.

RUNX1 DNA-binding mutants, associated with minimally differentiated acute myelogenous leukemia, disrupt myeloid differentiation.

Genome-wide resources of endoribonuclease-prepared short interfering RNAs for specific loss-of-function studies.

Tandem affinity purification of functional TAP-tagged proteins from human cells.

HIV-1 proviral DNA excision using an evolved recombinase.

Genome-scale RNAi profiling of cell division in human tissue culture cells.

BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.

Systems biology of mammalian cell division.

Three LIF-dependent signatures and gene clusters with atypical expression profiles, identified by transcriptome studies in mouse ES cells and early derivatives.

The symplekin/ZONAB complex inhibits intestinal cell differentiation by the repression of AML1/Runx1.

A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.

Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division.

HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.

Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice.

Engineering DNA processing enzymes for the postgenomic era.

The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation.

SKAnking with Ska3: essential role of Ska3 in cell division revealed by combined phenotypic profiling.

A domesticated transposon mediates the effects of a single-nucleotide polymorphism responsible for enhanced muscle growth.

Systems survey of endocytosis by multiparametric image analysis.

Systematic analysis of human protein complexes identifies chromosome segregation proteins.

PhenoFam-gene set enrichment analysis through protein structural information.

SeLOX--a locus of recombination site search tool for the detection and directed evolution of site-specific recombination systems.

In vitro evolution and analysis of HIV-1 LTR-specific recombinases.

A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.

High-throughput RNAi screening in mammalian cells with esiRNAs.

Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells.

From RNAi Screens to Molecular Function in Embryonic Stem Cells.

RAD21 cooperates with pluripotency transcription factors in the maintenance of embryonic stem cell identity.

A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.

An RNA interference phenotypic screen identifies a role for FGF signals in colon cancer progression.

Ribosome biogenesis and p53: who is regulating whom?

Combined RNAi and localization for functionally dissecting long noncoding RNAs.