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MISSION esiRNA für RNAi-Screening in Säugerzellen

16.1K Views

15:31 min

May 12th, 2010

DOI :

10.3791/2008-v

May 12th, 2010


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Cellular Biology

Kapitel in diesem Video

0:00

Title

2:22

Use of High-Quality MISSION esiRNA Libraries for RNAi Screening

3:49

Optimizing Transfection Prior to Screening

1:43

Introduction

5:28

Setting Up the Assay and Preparing for the Primary Screen

9:34

The Primary Screen

12:03

Secondary Screen of the Selected Genes for Hit Validation

13:40

Representative Results Screening for Mammalian Cell Cycle Genes Using MISSION esiRNA

14:43

Conclusion

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